Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand.
Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Andrologia. 2021 Mar;53(2):e13911. doi: 10.1111/and.13911. Epub 2020 Nov 26.
Dolichandrone serrulata flower (DSF) has been believed to reduce blood glucose in hyperglycaemic persons with sub-fertility but its effect on improvement of male reproductive impairment has never been elucidated scientifically. This study attempted to investigate the hypoglycaemic effects of DSF on male reproductive damages in type 2 diabetes mellitus (T2DM) rats. Adult Sprague Dawley rats were divided into four groups (control, T2DM, DSF200 + T2DM and DSF600 + T2DM; n = 10/each). Control rats received low-fat diet for 14 days before saline injection while streptozocin (50 mg/kg BW) induced T2DM groups received high-fat diet and were orally administered with DSF (200 and 600 mg/kg BW) for 28 days. At the end, fasted blood glucose (FBG), malondialdehyde (MDA), testosterone, sperm quality, histology and protein expressions were examined. The result showed that DSF decreased high FBG and testicular MDA and increased testosterone levels of T2DM-treated rats. Low-sperm quality and histological malfunction were ameliorated in DSF-treated group. There was significant decrease in the expression of androgen receptor, heat-shock 70 and steroidogenic acute regulatory proteins of T2DM-treated rats. Our study demonstrated changes of six bands (116, 51, 45, 39, 35 and 29 kDas) of tyrosine-phosphorylated proteins. In conclusion, DSF could reduce the FBGand ameliorate the reproductive damages in male T2DM rats.
密蒙花（Dolichandrone serrulata flower，DSF）被认为可以降低血糖水平，改善生育能力低下的糖尿病患者的血糖水平，但它对改善男性生殖损伤的作用从未得到科学阐明。本研究试图探讨密蒙花对 2 型糖尿病（type 2 diabetes mellitus，T2DM）大鼠生殖损伤的降血糖作用。成年 Sprague Dawley 大鼠分为 4 组（对照组、T2DM 组、DSF200+T2DM 组和 DSF600+T2DM 组；n=10/组）。对照组大鼠在注射生理盐水前接受低脂饮食 14 天，而链脲佐菌素（50mg/kg BW）诱导的 T2DM 组接受高脂饮食，并口服 DSF（200 和 600mg/kg BW）28 天。最后，检测空腹血糖（fasted blood glucose，FBG）、丙二醛（malondialdehyde，MDA）、睾酮、精子质量、组织学和蛋白质表达。结果表明，密蒙花降低了 T2DM 大鼠的高 FBG 和睾丸 MDA，增加了睾酮水平。密蒙花治疗组大鼠精子质量低和组织学功能障碍得到改善。T2DM 大鼠的雄激素受体、热休克蛋白 70 和甾体急性调节蛋白的表达显著下降。我们的研究显示了酪氨酸磷酸化蛋白的 6 条带（116、51、45、39、35 和 29kDa）的变化。综上所述，密蒙花可降低 FBG，改善雄性 T2DM 大鼠的生殖损伤。
