Poasakate Anuson, Maneesai Putcharawipa, Chiangsaen Petcharat, Bunbupha Sarawoot, Settheetham-Ishida Wannapa, Pakdeechote Poungrat
Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand.
Andrologia. 2021 Mar;53(2):e13917. doi: 10.1111/and.13917. Epub 2020 Nov 26.
The effects of a Cratoxylum formosum (Jack) Dyer ssp. (CF) extract on testicular damage were assessed in hypertensive rats. N -nitro-L-arginine methyl ester hydrochloride (L-NAME; 40 mg kg day ) was administered for 5 weeks to induce hypertension in male Sprague-Dawley rats, and treated with CF extract (100, 300 or 500 mg kg day ) or sildenafil (5 mg kg day ) during the final 2 weeks (n = 8/group). Biochemical components of the CF extract were identified and mainly contained phenolic compounds. The CF extract significantly reduced systolic blood pressure and alleviated impaired sperm quality and seminiferous tubular morphology in hypertensive rats. CF extract restored reduced serum testosterone and protein expression of steroidogenic acute regulatory protein (StAR), nuclear factor erythroid-related factor 2 (Nrf2), and haem oxygenase 1 (HO-1) in L-NAME rats. Hypertensive rats presented decreased antioxidant enzyme activities, and increased testicular and plasma malondialdehyde (MDA) levels and superoxide production, all of which were normalised by CF extract. Furthermore, endothelial nitric oxide synthase (eNOS) expression in testicular tissue and plasma nitrate/nitrite levels were restored in hypertensive rats administered CF extract. Conclusion: CF extract alleviated testicular damage in hypertensive rats. Potential molecular mechanisms may involve suppression of oxidative stress and restoration of StAR, Nrf2, HO-1 and eNOS expression in hypertensive rats.
在高血压大鼠中评估了台湾黄牛木（Cratoxylum formosum (Jack) Dyer ssp.，CF）提取物对睾丸损伤的影响。给雄性Sprague-Dawley大鼠连续5周给予盐酸N-硝基-L-精氨酸甲酯（L-NAME，40 mg/kg·天）以诱导高血压，并在最后2周用CF提取物（100、300或500 mg/kg·天）或西地那非（5 mg/kg·天）进行治疗（每组n = 8）。对CF提取物的生化成分进行了鉴定，其主要含有酚类化合物。CF提取物显著降低了高血压大鼠的收缩压，并改善了精子质量受损和生精小管形态异常的情况。CF提取物使L-NAME大鼠中降低的血清睾酮以及类固醇生成急性调节蛋白（StAR）、核因子红细胞相关因子2（Nrf2）和血红素加氧酶1（HO-1）的蛋白表达恢复正常。高血压大鼠的抗氧化酶活性降低，睾丸和血浆丙二醛（MDA）水平升高以及超氧化物生成增加，而CF提取物使所有这些指标均恢复正常。此外，给予CF提取物的高血压大鼠睾丸组织中的内皮型一氧化氮合酶（eNOS）表达和血浆硝酸盐/亚硝酸盐水平得以恢复。结论：CF提取物减轻了高血压大鼠的睾丸损伤。潜在的分子机制可能涉及抑制氧化应激以及恢复高血压大鼠中StAR、Nrf2、HO-1和eNOS的表达。
