Pharmacology Department, National Research Centre, Giza, Egypt.
Chemistry Department, College of Science, King Khalid University, Abha, Saudi Arabia.
Oxid Med Cell Longev. 2017;2017:9460653. doi: 10.1155/2017/9460653. Epub 2017 Oct 22.
This study aimed to evaluate the antihypertensive efficacy of a new combination therapy of and extracts (2 : 1; Roselle-Olive), using N(G)-nitro-L-arginine-methyl ester- (L-NAME-) induced hypertensive model. Rats received L-NAME (50 mg/kg/day, orally) for 4 weeks. Concurrent treatment with Roselle-Olive (500, 250, and 125 mg/kg/day for 4 weeks) resulted in a dose-dependent decrease in both systolic and diastolic blood pressure, reversed the L-NAME-induced suppression in serum nitric oxide (NO), and improved liver and kidney markers, lipid profile, and oxidative status. Furthermore, Roselle-Olive significantly lowered the elevated angiotensin-converting enzyme activity (ACE) and showed a marked genoprotective effect against oxidative DNA damage in hypertensive rats. Roselle-Olive ameliorated kidney and heart lesions and reduced aortic media thickness. Real-time PCR and immunohistochemistry showed an enhanced endothelial nitric oxide synthase (eNOS) gene and protein expression in both heart and kidney of Roselle-Olive-treated rats. To conclude, our data revealed that Roselle-Olive is an effective combination in which and synergistically act to control hypertension. These effects are likely to be mediated by antioxidant and genoprotective actions, ACE inhibition, and eNOS upregulation by Roselle-Olive constituents. These findings provide evidences that Roselle-Olive combination affords efficient antihypertensive effect with a broad end-organ protective influence.
本研究旨在评估新的组合疗法（ 和 提取物，比例为 2:1；洛神花-橄榄）对 N(G)-硝基-L-精氨酸甲酯（L-NAME）诱导的高血压模型的降压效果。大鼠接受 L-NAME（50mg/kg/天，口服）治疗 4 周。同时给予洛神花-橄榄（500、250 和 125mg/kg/天，连续 4 周）治疗可剂量依赖性地降低收缩压和舒张压，逆转 L-NAME 引起的血清一氧化氮（NO）抑制，并改善肝肾功能标志物、脂质谱和氧化状态。此外，洛神花-橄榄显著降低了升高的血管紧张素转换酶（ACE）活性，并对高血压大鼠的氧化 DNA 损伤表现出明显的基因保护作用。洛神花-橄榄改善了肾脏和心脏病变，并减少了主动脉中层厚度。实时 PCR 和免疫组织化学显示，治疗组大鼠心脏和肾脏的内皮型一氧化氮合酶（eNOS）基因和蛋白表达增强。综上所述，我们的数据表明，洛神花-橄榄是一种有效的组合，其中 和 协同作用来控制高血压。这些作用可能是通过抗氧化和基因保护作用、ACE 抑制以及洛神花-橄榄成分上调 eNOS 来介导的。这些发现提供了证据，表明洛神花-橄榄组合具有有效的降压作用，并对广泛的终末器官具有保护作用。
