Institut Jacques Monod (Université de Paris/CNRS) 15 rue H.Brion 75205 Paris cedex 13, France.
Res Microbiol. 2020 Dec;171(8):287-289. doi: 10.1016/j.resmic.2020.11.004. Epub 2020 Nov 25.
The Escherichia coli chromosome is a circular double helix. DNA polymerase, therefore, cannot use it directly as a template for polymerization until it has first been unwound. The DnaA protein opens the chromosome at a unique and specific site (oriC), which allows the polymerase to begin DNA replication. François Jacob and Sydney Brenner predicted the existence of the initiator protein, DnaA, back in the early 1960s. In order to demonstrate the existence of the hypothetical initiator, identification and isolation of dnaA mutants were undertaken. In the following, I recount, in a historical setting, the original research done on the identification and isolation of dnaA mutants.
大肠杆菌染色体是一个圆形的双螺旋。因此，DNA 聚合酶不能直接将其用作聚合的模板，除非它首先被解开。DnaA 蛋白在一个独特和特定的位点（oriC）打开染色体，这使得聚合酶能够开始 DNA 复制。弗朗索瓦·雅各布和悉尼·布伦纳早在 20 世纪 60 年代就预测了起始蛋白 DnaA 的存在。为了证明假设的起始蛋白的存在，进行了 dnaA 突变体的鉴定和分离。在下面，我将在历史背景下讲述关于 dnaA 突变体的鉴定和分离的原始研究。
