Menikpurage Inoka P, Woo Kristin, Mera Paola E
Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Front Microbiol. 2021 Jun 1;12:662317. doi: 10.3389/fmicb.2021.662317. eCollection 2021.
In bacteria, DnaA is the most conserved DNA replication initiator protein. DnaA is a DNA binding protein that is part of the AAA+ ATPase family. In addition to initiating chromosome replication, DnaA can also function as a transcription factor either as an activator or repressor. The first gene identified to be regulated by DnaA at the transcriptional levels was . DnaA has been shown to regulate genes involved in a variety of cellular events including those that trigger sporulation, DNA repair, and cell cycle regulation. DnaA's dual functions (replication initiator and transcription factor) is a potential mechanism for DnaA to temporally coordinate diverse cellular events with the onset of chromosome replication. This strategy of using chromosome replication initiator proteins as regulators of gene expression has also been observed in archaea and eukaryotes. In this mini review, we focus on our current understanding of DnaA's transcriptional activity in various bacterial species.
在细菌中，DnaA是最保守的DNA复制起始蛋白。DnaA是一种DNA结合蛋白，属于AAA+ATP酶家族。除了启动染色体复制外，DnaA还可以作为转录因子发挥作用，既可以作为激活剂，也可以作为抑制剂。第一个被确定在转录水平受DnaA调控的基因是 。已证明DnaA可调控参与多种细胞事件的基因，包括那些触发孢子形成、DNA修复和细胞周期调控的基因。DnaA的双重功能（复制起始因子和转录因子）是DnaA在时间上协调多种细胞事件与染色体复制起始的潜在机制。在古细菌和真核生物中也观察到了这种利用染色体复制起始蛋白作为基因表达调节因子的策略。在本综述中，我们重点关注目前对DnaA在各种细菌物种中转录活性的理解。
