Aquaculture Production and Environment Division, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India.
Aquaculture Production and Environment Division, ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India.
Anim Reprod Sci. 2021 Jan;224:106650. doi: 10.1016/j.anireprosci.2020.106650. Epub 2020 Nov 12.
In the present study, there was assessment of effects of gonadotropin treatments on broodstock maturation, induced breeding, and spawning outcomes of striped snakehead in captivity. The striped snakehead (n = 128) were equally distributed in four concrete tanks (15 m) and hormone implants (500 IU human chorionic gonadotropin (hCG)/kg body weight) were inserted intramuscularly and striped snakehead broodstock administered this treatment were confined in two tanks and striped snakehead of a non-implanted group were confined in two tanks. The hormone implanted striped snakehead had a greater (P < 0.05) gonadosomatic index (GSI) and oocyte diameter in comparison to non-implanted striped snakehead. In a subsequent experiment, hCG and carp pituitary homogenate (CPH) were evaluated for inducing breeding. Dosages of hCG used were, 2,000 (T), 3000 (T), and 4000 (T) IU hCG/kg body weight of females. Dosages of CPH were, 20 (T), 30 (T), and 40 (T) mg CPH/kg body weight of females. Males were administered 0.75 of the dosage administered to females. The values for reproductive variables were estimated. Fertilization (89.0 ± 3.0 %) and hatching (92.0 ± 1.0 %) rates were greater (P < 0.05) in the T group of implanted striped snakehead. Relative fecundity (19,023 ± 2211), as well as fertilization (96.2 ± 2.4 %), and hatching (96.6 ± 1.7 %) rates were greater in the T group of the implanted striped snakehead. The results from the present study indicate broodstock treated with gonadotropins had greater spawning outcomes which might facilitate mass scale breeding and fertilized egg as well as juvenile production of striped snakehead in captivity.
在本研究中，评估了促性腺激素处理对养殖条纹石斑鱼亲鱼成熟、诱导繁殖和产卵结果的影响。将 128 条条纹石斑鱼（n = 128）平均分配到四个混凝土池中（15m），并将激素植入物（500IU 人绒毛膜促性腺激素（hCG）/kg 体重）肌肉内插入，接受这种处理的条纹石斑鱼亲鱼被限制在两个池中，而未植入组的条纹石斑鱼被限制在两个池中。与未植入的条纹石斑鱼相比，植入激素的条纹石斑鱼具有更高的（P<0.05）性腺指数（GSI）和卵母细胞直径。在随后的实验中，评估了 hCG 和鲤鱼脑垂体匀浆（CPH）诱导繁殖的效果。使用的 hCG 剂量分别为 2000（T）、3000（T）和 4000（T）IU hCG/kg 雌性体重。CPH 的剂量分别为 20（T）、30（T）和 40（T）mg CPH/kg 雌性体重。雄性给予雌性剂量的 0.75。估计了生殖变量的值。受精率（89.0±3.0%）和孵化率（92.0±1.0%）较高（P<0.05）在植入条纹石斑鱼的 T 组中。相对繁殖力（19023±2211）、受精率（96.2±2.4%）和孵化率（96.6±1.7%）在植入条纹石斑鱼的 T 组中更高。本研究的结果表明，用促性腺激素处理的亲鱼具有更高的产卵效果，这可能有助于大规模繁殖和受精卵以及养殖条纹石斑鱼的幼鱼生产。
