Department of Biomedical Sciences, University of Padova, Padova, Italy.
Department of Biology, University of Padova, Padova, Italy.
Nat Commun. 2020 Nov 27;11(1):6069. doi: 10.1038/s41467-020-19892-6.
Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The lack of tools to finely image inter-organelle proximity hindered our understanding on how these subcellular communication hubs mediate and regulate cell homeostasis. We develop an improved and expanded palette of split-GFP-based contact site sensors (SPLICS) for the detection of single and multiple organelle contact sites within a scalable distance range. We demonstrate their flexibility under physiological conditions and in living organisms.
已经记录了几乎所有已知细胞器之间的膜接触位点，在过去几十年中，由于它们对细胞基本活动和许多人类疾病的微妙理解的重要性，它们的研究得到了推动。缺乏精细成像细胞器接近度的工具阻碍了我们对这些亚细胞通讯枢纽如何介导和调节细胞内稳态的理解。我们开发了一套经过改进和扩展的基于分裂 GFP 的接触位点传感器 (SPLICS) ，用于在可扩展的距离范围内检测单个和多个细胞器接触位点。我们证明了它们在生理条件下和活体生物中的灵活性。
