Venetian Institute of Molecular Medicine, Department of Biology, University of Padua, Padua, Italy.
Telethon Institute of Genetics and Medicine, Pozzuoli, Naples, Italy.
Nat Commun. 2019 Mar 20;10(1):1287. doi: 10.1038/s41467-019-09253-3.
Close proximities between organelles have been described for decades. However, only recently a specific field dealing with organelle communication at membrane contact sites has gained wide acceptance, attracting scientists from multiple areas of cell biology. The diversity of approaches warrants a unified vocabulary for the field. Such definitions would facilitate laying the foundations of this field, streamlining communication and resolving semantic controversies. This opinion, written by a panel of experts in the field, aims to provide this burgeoning area with guidelines for the experimental definition and analysis of contact sites. It also includes suggestions on how to operationally and tractably measure and analyze them with the hope of ultimately facilitating knowledge production and dissemination within and outside the field of contact-site research.
细胞器之间的紧密接近已经被描述了几十年。然而，直到最近，一个专门研究细胞器在膜接触位点处进行通讯的特定领域才得到广泛认可，吸引了来自细胞生物学多个领域的科学家。这种多样性的方法需要一个统一的词汇来描述这个领域。这样的定义将有助于为这个领域奠定基础，简化沟通并解决语义争议。这篇由该领域专家小组撰写的观点文章旨在为接触位点的实验定义和分析提供指导方针。它还包括了关于如何操作和可处理地测量和分析它们的建议，希望最终能够促进接触位点研究领域内外的知识生产和传播。
