Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 1176, 165 21, Praha 6, Suchdol, Czech Republic.
VSN International, Hamel Hamstead, UK.
Sci Rep. 2020 Nov 27;10(1):20789. doi: 10.1038/s41598-020-76900-x.
Norway spruce has a broad natural distribution range, which results in a substantial variety of its physiological and genetic variation. There are three distinct altitudinal ecotypes described in this tree species. The physiological optimum of each ecotype may be shifted due to ongoing climate change, especially in traits associated with water demand that might be crucial for adaptation. Dehydrins are proteins that help to mitigate the adverse effects of dehydration. Dehydrin gene expression patterns appeared to be a suitable marker for plant stress assessment. Genetically determined differences in response between individuals and populations were formerly studied, however, mainly in controlled conditions. We evaluated ecotypic variation in dehydrin gene expression in a clonal bank comprised of all three ecotypes. A genetic relationship among targeted trees was uncovered utilizing GBS (Genotyping by Sequencing) platform. We sampled 4-6 trees of each ecotype throughout 15 months period. Subsequently, we assessed the RNA expression of dehydrin genes by qRT-PCR. For this study, we deliberately selected dehydrins from different categories. Our findings detected significant differences among ecotypes in dehydrin expression. The association of recorded climatic variables and individual gene expression across the study period was evaluated and revealed, for certain genes, a correlation between dehydrin gene expression and precipitation, temperature, and day-length.
挪威云杉分布范围广泛，导致其在生理和遗传方面存在很大的变异性。在该树种中描述了三种截然不同的海拔生态型。由于持续的气候变化，每个生态型的生理最佳状态可能会发生变化，特别是与水分需求相关的特征，这可能对适应至关重要。脱水素是有助于减轻脱水不利影响的蛋白质。脱水素基因表达模式似乎是植物应激评估的合适标志物。以前曾研究过个体和种群之间响应的遗传决定差异，但主要是在受控条件下进行的。我们在由所有三种生态型组成的克隆库中评估了脱水素基因表达的生态型变异。利用 GBS（测序基因分型）平台揭示了目标树木之间的遗传关系。我们在 15 个月的时间内对每个生态型的 4-6 棵树进行了采样。随后，我们通过 qRT-PCR 评估了脱水素基因的 RNA 表达。在这项研究中，我们有意从不同类别中选择脱水素。我们的研究结果发现，在脱水素表达方面，生态型之间存在显著差异。评估了记录的气候变量与整个研究期间个体基因表达之间的关联，并发现某些基因的脱水素基因表达与降水、温度和日照时间之间存在相关性。
