Rico Patricia, Rodrigo-Navarro Aleixandre, Sánchez Pérez Laura, Salmeron-Sanchez Manuel
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain.
Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain.
Commun Biol. 2020 Nov 27;3(1):717. doi: 10.1038/s42003-020-01449-4.
The intrinsic properties of mesenchymal stem cells (MSCs) make them ideal candidates for tissue engineering applications. Efforts have been made to control MSC behavior by using material systems to engineer synthetic extracellular matrices and/or include soluble factors in the media. This work proposes a simple approach based on ion transporter stimulation to determine stem cell fate that avoids the use of growth factors. Addition of borax alone, transported by the NaBC1-transporter, enhanced MSC adhesion and contractility, promoted osteogenesis and inhibited adipogenesis. Stimulated-NaBC1 promoted osteogenesis via the BMP canonical pathway (comprising Smad1/YAP nucleus translocation and osteopontin expression) through a mechanism that involves simultaneous NaBC1/BMPR1A and NaBC1/αβ/αβ co-localization. We describe an original function for NaBC1 transporter, besides controlling borate homeostasis, capable of stimulating growth factor receptors and fibronectin-binding integrins. Our results open up new biomaterial engineering approaches for biomedical applications by a cost-effective strategy that avoids the use of soluble growth factors.
间充质干细胞（MSC）的内在特性使其成为组织工程应用的理想候选者。人们已努力通过使用材料系统来构建合成细胞外基质和/或在培养基中添加可溶性因子来控制MSC的行为。这项工作提出了一种基于离子转运体刺激来确定干细胞命运的简单方法，该方法避免使用生长因子。单独添加由NaBC1转运体转运的硼砂，可增强MSC的黏附性和收缩性，促进成骨作用并抑制脂肪生成。受刺激的NaBC1通过涉及NaBC1/BMPR1A和NaBC1/αβ/αβ同时共定位的机制，经由BMP经典途径（包括Smad1/YAP核转位和骨桥蛋白表达）促进成骨作用。我们描述了NaBC1转运体的一种原始功能，除了控制硼酸盐稳态外，它还能够刺激生长因子受体和纤连蛋白结合整合素。我们的结果通过一种避免使用可溶性生长因子的经济有效策略，为生物医学应用开辟了新的生物材料工程方法。
