Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, Istanbul, Turkey.
Health Institutes of Turkey, Turkish Cancer Institute, Istanbul, Turkey.
Biol Trace Elem Res. 2019 Apr;188(2):384-392. doi: 10.1007/s12011-018-1428-5. Epub 2018 Jul 6.
Over the past years, adipose tissue has become an invaluable source of mesenchymal stem cells (MSCs) due to development of improved isolation methodologies. In a recent work, our group established a primary culture of human adipose-derived stem cells (hADSCs), which were characterized for their stem cell characteristics in detail and studied their myogenic differentiation potential in presence of boron. In the current study, we focused on the effects of a boron-containing compound, sodium pentaborate pentahydrate (NaB), on the adipogenic differentiation of hADSCs. Incorporation of boron in various chemical derivates has been a novel interest in drug-discovery attempts due to increasing number of reports on their anticancer, antibacterial, antiviral, and antifungal activities. In this report, a striking suppressive activity of boron on adipogenic differentiation of hADSCs is observed in a dose-dependent manner. Higher concentrations of NaB (20, 50, and 100 μg/mL (68, 170 and 340 μM)) resulted in a progressive decrease of lipid deposition, suppressed master regulators of adipogenesis transcriptional programming at the mRNA and protein levels, while having no evident cytotoxicity on the cells. The findings of this study are encouraging to undertake further investigations on potential beneficial effects boron in terms of its impact on normal and dysfunctional adipose biology. In that respect, these results pave the path to evaluate boron-based compounds in prevention and treatment of obesity which is a modern age pandemic that is predominant worldwide and found in strong association with comorbidities, including type 2 diabetes, hypertension, cardiovascular disease, cancers, and others."
在过去的几年中，由于改进的分离方法的发展，脂肪组织已成为间充质干细胞（MSCs）的宝贵来源。在最近的一项工作中，我们的小组建立了人脂肪来源干细胞（hADSCs）的原代培养物，详细表征了其干细胞特性，并研究了它们在硼存在下的成肌分化潜能。在当前的研究中，我们专注于含硼化合物，五硼酸钠五水合物（NaB）对 hADSCs 脂肪生成分化的影响。由于越来越多的关于硼的抗癌、抗菌、抗病毒和抗真菌活性的报道，将硼掺入各种化学衍生物中已成为药物发现尝试中的新兴趣。在本报告中，观察到硼以剂量依赖性方式对 hADSCs 脂肪生成分化具有显著的抑制作用。较高浓度的 NaB（20、50 和 100μg/mL（68、170 和 340μM））导致脂质沉积逐渐减少，抑制脂肪生成转录编程的主调控因子在 mRNA 和蛋白质水平上，而对细胞没有明显的细胞毒性。本研究的发现令人鼓舞，可进一步研究硼在正常和功能失调的脂肪生物学方面的潜在有益作用。在这方面，这些结果为评估基于硼的化合物在预防和治疗肥胖症铺平了道路，肥胖症是一种现代时代的大流行病，在全球范围内普遍存在，并与包括 2 型糖尿病、高血压、心血管疾病、癌症等在内的多种合并症密切相关。
