Hou Zhuoni, Li Yuanyuan, Su Feng, Chen Jipeng, Zhang Xiaodan, Xu Ling, Yang Dongfeng, Liang Zongsuo
The Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, 18 Chao Wang Road, Hangzhou, 310014, China.
Planta. 2020 Nov 27;253(1):2. doi: 10.1007/s00425-020-03506-y.
Methyl jasmonate promotes the synthesis of rosmarinic acid in Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib, and it promotes the latter more strongly. Salvia miltiorrhiza Bunge (SMB) is a traditional Chinese medicinal material, its water-soluble phenolic acid component rosmarinic acid has very important medicinal value. Salvia castanea f. tomentosa Stib (SCT) mainly distributed in Nyingchi, Tibet. Its pharmacological effects are similar to SMB, but its rosmarinic acid is significantly higher than the former. Methyl jasmonate (MJ) as an inducer can induce the synthesis of phenolic acids in SMB and SCT. However, the role of MJ on rosmarinic acid in SMB is controversial. Therefore, this study used SMB and SCT hair root as an experimental material and MJ as a variable. On one hand, exploring the controversial reports in SMB; on the other hand, comparing the differences in the mechanism of action of MJ on the phenolic acids in SMB and SCT. The content of related metabolites and the expression of key genes in the synthesis pathway of rosmarinic acid was analyzed by H-NMR combined with qRT-PCR technology. Our research has reached the following conclusions: first of all, MJ promotes the accumulation of rosmarinic acid and related phenolic acids in the metabolic pathways of SMB and SCT. After MJ treatment, the content of related components and gene expression are increased. Second, compared to SMB, SCT has a stronger response to MJ. It is speculated that the different responses of secondary metabolism-related genes to MJ may lead to different metabolic responses of salvianolic acid between the two.
茉莉酸甲酯促进丹参和绒毛栗色鼠尾草中迷迭香酸的合成，且对后者的促进作用更强。丹参是一种传统中药材，其水溶性酚酸成分迷迭香酸具有非常重要的药用价值。绒毛栗色鼠尾草主要分布于西藏林芝。其药理作用与丹参相似，但其迷迭香酸含量明显高于丹参。茉莉酸甲酯作为诱导剂可诱导丹参和绒毛栗色鼠尾草中酚酸的合成。然而，茉莉酸甲酯对丹参中迷迭香酸的作用存在争议。因此，本研究以丹参和绒毛栗色鼠尾草毛状根为实验材料，以茉莉酸甲酯为变量。一方面，探究丹参中存在争议的报道；另一方面，比较茉莉酸甲酯对丹参和绒毛栗色鼠尾草中酚酸作用机制的差异。采用氢核磁共振波谱结合定量逆转录聚合酶链反应技术分析迷迭香酸合成途径中相关代谢产物的含量和关键基因的表达。本研究得出以下结论：首先，茉莉酸甲酯促进丹参和绒毛栗色鼠尾草代谢途径中迷迭香酸及相关酚酸的积累。经茉莉酸甲酯处理后，相关成分含量和基因表达均增加。其次，与丹参相比，绒毛栗色鼠尾草对茉莉酸甲酯的反应更强。推测次生代谢相关基因对茉莉酸甲酯的不同反应可能导致二者之间丹酚酸的代谢反应不同。
