间接混合抗球蛋白反应（MAR）作为抗精子抗体的一种筛查方法。II. 临床研究。

Indirect mixed antiglobulin reaction (MAR) as a screening procedure for antisperm antibodies. II. Clinical studies.

作者信息

Meinertz H

机构信息

Institute of Medical Microbiology, University of Aarhus, Denmark.

出版信息

Am J Reprod Immunol Microbiol. 1987 Nov;15(3):101-5. doi: 10.1111/j.1600-0897.1987.tb00165.x.

DOI:10.1111/j.1600-0897.1987.tb00165.x

Abstract

In this study the question was raised whether the indirect mixed antiglobulin reaction (MAR) using donor spermatozoa could substitute the direct MAR requiring motile spermatozoa in the patient's ejaculate in the screening for antisperm antibodies. Serum and seminal plasma from 48 men selected on the basis of the results in the direct MAR were tested by the indirect MAR. Both tests were carried out for IgG and IgA antisperm antibodies. A statistically significant correlation was found both between the sperm-bound IgG and the free IgG antisperm antibodies in serum as well as in seminal plasma and between sperm-bound IgA and the free IgA antisperm antibodies in seminal plasma whereas no correlation was found between the sperm-bound IgA and the free IgA antisperm antibodies in serum. This finding strongly supports the concept that the major part of the IgA antisperm antibodies in the ejaculate are locally produced.

摘要

在本研究中，提出了一个问题，即在抗精子抗体筛查中，使用供体精子的间接混合抗球蛋白反应（MAR）是否可以替代需要患者射精中活动精子的直接MAR。根据直接MAR的结果选取了48名男性的血清和精浆，通过间接MAR进行检测。针对IgG和IgA抗精子抗体进行了两种检测。在血清和精浆中，精子结合的IgG与游离的IgG抗精子抗体之间以及精子结合的IgA与精浆中游离的IgA抗精子抗体之间均发现了具有统计学意义的相关性，而在血清中精子结合的IgA与游离的IgA抗精子抗体之间未发现相关性。这一发现有力地支持了射精中IgA抗精子抗体的主要部分是局部产生的这一概念。