Key Laboratory of Animal Genetics, Breeding and Reproduction of the Ministry of Agriculture & Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing 100193, China.
Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, Guilan, Iran.
Poult Sci. 2020 Dec;99(12):6371-6377. doi: 10.1016/j.psj.2020.09.002. Epub 2020 Sep 15.
We previously reported that blue eggshell color in chickens is associated with a partial endogenous retroviral (EAV-HP) insertion in the promoter region of the solute carrier organic anion transporter family member 1B3 (SLCO1B3) gene. The EAV-HP sequence includes numerous regulatory elements, which may modulate the expression of adjacent genes. To determine whether this insertion influences the expression of neighboring genes, we screened the expression of solute carrier organic anion transporter family members 1C1, 1B1 (SLCO1C1, SLCO1B1), and SLCO1B3 in 13 and 10 tissues from female and male Yimeng chickens, respectively. We observed that the insertion only significantly modulated the expression of SLCO1B3 and did not majorly affect that of SLCO1C1 and SLCO1B1. High expression of SLCO1B3 was detected in the shell gland, magnum, isthmus, and vagina of the oviduct in female blue-eggshell chickens. We also observed ectopic expression of SLCO1B3 in the testes of male chickens. SLCO1B3 is typically highly expressed in the liver; however, the EAV-HP insertion significantly reduces SLCO1B3 expression. As a liver-specific transporter, a reduction in the expression of SLCO1B3 may affect liver metabolism, particularly that of bile acids. We also detected higher ectopic expression of SLCO1B3 in the lungs of birds heterozygous for the EAV-HP insertion than in homozygous genotypes. In conclusion, we confirmed that the EAV-HP insertion modifies SLCO1B3 expression, and showed, for the first time, similar expression profile of this gene in all parts of the oviduct in females and testis in males. We also observed different levels of SLCO1B3 expression in the liver, which were associated with the EAV-HP insertion, and significantly higher expression in the lungs of birds with heterozygous genotype. The effects of these changes in the SLCO1B3 expression pattern on the function of the tissues warrant further investigation.
我们之前报道过，鸡的蓝色蛋壳颜色与溶质载体有机阴离子转运家族成员 1B3（SLCO1B3）基因启动子区域的部分内源性逆转录病毒（EAV-HP）插入有关。EAV-HP 序列包含许多调节元件，这些元件可能调节相邻基因的表达。为了确定这种插入是否影响邻近基因的表达，我们筛选了 13 只雌性和 10 只雄性沂蒙鸡的 13 种和 10 种组织中溶质载体有机阴离子转运家族成员 1C1、1B1（SLCO1C1、SLCO1B1）和 SLCO1B3 的表达情况。我们观察到，该插入仅显著调节 SLCO1B3 的表达，而对 SLCO1C1 和 SLCO1B1 的表达影响不大。在产蛋鸡的蛋壳腺、子宫角、峡部和阴道中检测到 SLCO1B3 的高表达。我们还观察到 SLCO1B3 在雄性鸡睾丸中的异位表达。SLCO1B3 通常在肝脏中高度表达；然而，EAV-HP 插入显著降低了 SLCO1B3 的表达。作为一种肝脏特异性转运体，SLCO1B3 表达的减少可能会影响肝脏代谢，尤其是胆汁酸的代谢。我们还检测到，在 EAV-HP 插入杂合子鸟类的肺部中，SLCO1B3 的异位表达高于纯合子基因型。总之，我们证实了 EAV-HP 插入会改变 SLCO1B3 的表达，并首次显示该基因在雌性所有输卵管部位和雄性睾丸中的表达模式相似。我们还观察到，肝脏中 SLCO1B3 的表达水平不同，这与 EAV-HP 插入有关，并且在杂合基因型鸟类的肺部中表达水平显著升高。需要进一步研究这些 SLCO1B3 表达模式变化对组织功能的影响。
