Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India.
Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India.
Int J Biol Macromol. 2021 Jan 1;166:9-17. doi: 10.1016/j.ijbiomac.2020.11.171. Epub 2020 Nov 26.
Statistical optimization of hydrolytic conditions for the production of fructooligosaccharides (FOSs) from pure inulin using Aspergillus tritici endoinulinase was carried out in a batch system. FOSs yield 99.19% was obtained under the optimized hydrolytic conditions i.e. inulin concentration (7.3%), enzyme load (65 IU), hydrolysis time (13 h) and agitation (100 rpm). The closeness of value of co-efficient of determination (R) to 1, good agreement between model's predicted and experimental values, low percentage error (<5%), high adequate precision (>4%) and F value (11,634.32), and low Lack of fit (0.60) of the designed model authenticates its fitness. High substrate concentration, low enzyme load and short hydrolysis span justifies efficiency of developed process for the preparation of FOSs from inulin using fungal endoinulinase. TLC chromatographic and densitometry studies confirmed the synthesis of short-chain length FOSs. FOSs preparation contained 33.85% GF2 (ketose), 24.50% GF3 (nystose), 7.26% GF4 (fructofuranosylnystose) and 33.58% FOSs of DP5-9.
采用黑曲霉内切菊粉酶在分批体系中对从纯菊粉生产低聚果糖（FOSs）的水解条件进行了统计优化。在优化的水解条件下，即菊粉浓度（7.3%）、酶用量（65 IU）、水解时间（13 h）和搅拌速度（100 rpm）下，获得了 99.19%的 FOSs 产率。接近 1 的决定系数（R）值、模型预测值与实验值之间良好的一致性、低百分比误差（<5%）、高充分精度（>4%）和 F 值（11,634.32），以及低拟合不足（0.60）证明了设计模型的拟合度。高底物浓度、低酶用量和短水解时间证明了真菌内切菊粉酶用于从菊粉制备 FOSs 的开发工艺的效率。TLC 色谱和密度计研究证实了短链长度 FOSs 的合成。FOSs 制剂含有 33.85% GF2（酮糖）、24.50% GF3（棉子糖）、7.26% GF4（果呋喃基棉子糖）和 33.58% DP5-9 的 FOSs。
