Department of Clinical Laboratory, Affiliated Yancheng Hospital, School of Medicine, Southeast University, Yancheng, Jiangsu, China.
Department of Clinical Laboratory, Henan Provincial People's Hospital, Department of Clinical Laboratory of Central China Fuwai Hospital, Central China Fuwai Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China.
Microb Pathog. 2021 Mar;152:104651. doi: 10.1016/j.micpath.2020.104651. Epub 2020 Nov 27.
The presence of persisters causes recalcitrance to antibiotic treatment, and can be attributed to a fairly large number of clinically refractory infections in several species of bacteria. Many studies have explored this phenomenon, but the mechanisms remain poorly understood. In this study, we found that the deletion of fis, which encodes a key DNA-binding protein mediating various biological processes, significantly reduced persister formation in S. Typhi. Persister assays and glutamate determination analysis showed that Fis mediated Salmonella persistence through regulating glutamate metabolism. Additionally, glutamate incubation altered the expression of the stringent response regulatory genes, demonstrating that the stringent response was related to glutamate regulation by Fis. The findings revealed that glutamate metabolism regulated by Fis serves as a mechanism for persister formation in S. Typhi.
持久菌的存在导致抗生素治疗产生抗药性，这可以归因于临床上许多种细菌对多种抗生素产生抗药性。许多研究都探讨了这种现象，但机制仍不清楚。在这项研究中，我们发现 fis 的缺失，该基因编码一种关键的 DNA 结合蛋白，介导多种生物学过程，可显著减少伤寒沙门氏菌中的持久菌形成。持久菌试验和谷氨酸测定分析表明，Fis 通过调节谷氨酸代谢介导沙门氏菌的持续存在。此外，谷氨酸孵育改变了严格反应调控基因的表达，表明严格反应与 Fis 对谷氨酸的调节有关。研究结果表明，Fis 调节的谷氨酸代谢是伤寒沙门氏菌持久菌形成的一种机制。
