从人肠道中筛选替加环素耐药基因 tet(X)的方法。
A method for screening tigecycline-resistant gene tet(X) from human gut.
机构信息
Department of Clinical Laboratory, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China.
出版信息
J Glob Antimicrob Resist. 2021 Mar;24:29-31. doi: 10.1016/j.jgar.2020.11.010. Epub 2020 Nov 26.
OBJECTIVES
To develop an effective enrichment method for tet(X) detection, we performed PCR and Sanger sequencing to screen and confirm the presence of tet(X) gene.
METHODS
Species were identified by MALDI-TOF MS analysis. The minimum inhibitory concentrations (MICs) of common antibiotics were determined by broth microdilution and interpreted according to the CLSI guidelines and EUCAST breakpoints.
RESULTS
We obtained 29 (2.26%, 29/1284) tet(X4)-positive Escherichia coli, and 96.6% of those (28 isolates) exhibited resistance to tigecycline.
CONCLUSION
This specific screening strategy for functional tet(X) mediating tigecycline resistance will be useful to facilitate development and advancement of our knowledge of tet(X).
目的
为了开发一种有效的 tet(X)检测富集方法，我们进行了 PCR 和 Sanger 测序来筛选和确认 tet(X)基因的存在。
方法
通过 MALDI-TOF MS 分析鉴定菌种。采用肉汤微量稀释法测定常见抗生素的最小抑菌浓度（MIC），并根据 CLSI 指南和 EUCAST 折点进行解释。
结果
我们获得了 29 株 tet(X4)阳性大肠杆菌（2.26%，29/1284），其中 96.6%（28 株）对替加环素耐药。
结论
这种针对功能性 tet(X)介导替加环素耐药的特定筛选策略，将有助于促进我们对 tet(X)的认识的发展和进步。
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