脑瘫患儿血清微量元素及氨基酸谱分析。
Serum trace element and amino acid profile in children with cerebral palsy.
机构信息
Yaroslavl State University, 150003, Yaroslavl, Russia.
Yaroslavl State University, 150003, Yaroslavl, Russia.
出版信息
J Trace Elem Med Biol. 2021 Mar;64:126685. doi: 10.1016/j.jtemb.2020.126685. Epub 2020 Nov 12.
BACKGROUND
The existing data demonstrate that both trace elements and amino acids play a significant role in neurodevelopment and brain functioning. Certain studies have demonstrated alteration of micronutrient status in children with cerebral palsy, although multiple inconsistencies exist.
THE OBJECTIVE
of the present study was to assess serum trace element and mineral, as well as amino acid levels in children with cerebral palsy.
METHODS
71 children with cerebral palsy (39 boys and 32 girls, 5.7 ± 2.3 y.o.) and 84 healthy children (51 boys and 33 girls, 5.4 ± 2.3 y.o.) were enrolled in the present study. Serum trace element and mineral levels were assessed using inductively-coupled plasma mass-spectrometry (ICP-MS). Amino acid profile was evaluated by means of high-pressure liquid chromatography (HPLC).
RESULTS
Children with cerebral palsy are characterized by significantly lower Cu and Zn levels by 6% and 8%, whereas serum I concentration exceeded the control values by 7%. A tendency to increased serum Mn and Se levels was also observed in patients with cerebral palsy. Serum citrulline, leucine, tyrosine, and valine levels were 15 %, 23 %, 15 %, and 11 % lower than those in healthy controls. Nearly twofold lower levels of serum proline were accompanied by a 44 % elevation of hydroxyproline concentrations when compared to the control values. In multiple regression model serum I, Zn, and hydroxyproline levels were found to be independently associated with the presence of cerebral palsy. Correlation analysis demonstrated a significant correlation between Cu, Mn, Se, I, and Zn levels with hydroxyproline and citrulline concentrations.
CONCLUSION
The observed alterations in trace element and amino acid metabolism may contribute to neurological deterioration in cerebral palsy. However, the cross-sectional design of the study does not allow to estimate the causal trilateral relationships between cerebral palsy, altered trace element, and amino acid metabolism.
背景
现有数据表明，微量元素和氨基酸在神经发育和大脑功能中都起着重要作用。尽管存在多种不一致之处，但某些研究已经证明脑瘫儿童的微量营养素状态发生了改变。
目的
本研究旨在评估脑瘫儿童的血清微量元素和矿物质以及氨基酸水平。
方法
本研究纳入了 71 名脑瘫儿童（39 名男孩和 32 名女孩，5.7±2.3 岁）和 84 名健康儿童（51 名男孩和 33 名女孩，5.4±2.3 岁）。使用电感耦合等离子体质谱法（ICP-MS）评估血清微量元素和矿物质水平。通过高效液相色谱法（HPLC）评估氨基酸谱。
结果
脑瘫儿童的 Cu 和 Zn 水平分别低 6%和 8%，而血清 I 浓度比对照组高 7%。脑瘫儿童的血清 Mn 和 Se 水平也有升高的趋势。脑瘫儿童的血清瓜氨酸、亮氨酸、酪氨酸和缬氨酸水平分别比健康对照组低 15%、23%、15%和 11%。与对照组相比，血清脯氨酸水平低近两倍，同时羟脯氨酸浓度升高 44%。多元回归模型显示，血清 I、Zn 和羟脯氨酸水平与脑瘫的发生独立相关。相关性分析表明，Cu、Mn、Se、I 和 Zn 水平与羟脯氨酸和瓜氨酸浓度之间存在显著相关性。
结论
观察到的微量元素和氨基酸代谢的改变可能导致脑瘫患者的神经功能恶化。然而，该研究的横断面设计不允许评估脑瘫、微量元素和氨基酸代谢改变之间的因果三方关系。
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