College of Materials Science and Engineerging, Yangtze Normal University, Fuling, 408100, PR China.
College of Materials Science and Engineerging, Yangtze Normal University, Fuling, 408100, PR China.
Anal Chim Acta. 2020 May 22;1112:1-7. doi: 10.1016/j.aca.2020.03.054. Epub 2020 Apr 1.
The occurrence and development of hepatocellular carcinoma (HCC) is related to the level of trace elements. The mechanism of changes in trace element concentration and the identification of HCC risks associated with them are not clear. Therefore, an accurate and reliable approach for quantification of the level of trace elements in the serum of liver cancer patients is of great significance. In this study, we proposed a novel strategy for accurate determination of trace elements such as Mn, Fe, Ni, Cu, Zn, and Se in the serum of HCC patients based on inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). In the MS/MS mode, NO was used as the reaction gas, and an octopole reaction system (ORS) was employed to provide interference-free conditions and a sufficiently low limit of detection (LOD) for the determination of analytical elements. The internal standard elements with similar masses and similar mass spectrometry behavior were selected to correct the matrix effect. The LODs of Mn, Fe, Ni, Cu, Zn, and Se were as low as 0.013, 0.25, 0.010, 0.020, 0.17, and 0.008 μg L, respectively. Bovine serum (SRM 1598a) was adopted as the standard reference material to evaluate the accuracy of the method, and the developed method was successfully applied to determine the content of Mn, Fe, Cu, Zn, Ni, Se in serum from HCC patients. The obtained results were in agreement with that of sector field ICP-MS (SF-ICP-MS). The contents of Fe and Cu in the serum of HCC patients were higher compared to that of healthy controls, while the contents of Zn and Se were lower than that of healthy controls. Furthermore, no significant differences were observed in the contents of Mn and Ni in the serum of HCC patients and healthy controls.
肝细胞癌 (HCC) 的发生和发展与微量元素水平有关。微量元素浓度变化的机制以及与它们相关的 HCC 风险的识别尚不清楚。因此，准确可靠地定量检测肝癌患者血清中微量元素的水平具有重要意义。在本研究中，我们提出了一种基于电感耦合等离子体质谱串联质谱 (ICP-MS/MS) 准确测定 HCC 患者血清中 Mn、Fe、Ni、Cu、Zn 和 Se 等微量元素的新策略。在 MS/MS 模式下，使用 NO 作为反应气体，采用八极反应系统 (ORS) 提供无干扰条件和足够低的分析元素检测限 (LOD)。选择具有相似质量和相似质谱行为的内标元素来校正基质效应。Mn、Fe、Ni、Cu、Zn 和 Se 的 LOD 分别低至 0.013、0.25、0.010、0.020、0.17 和 0.008μg/L。采用牛血清 (SRM 1598a) 作为标准参考物质评估方法的准确性，并成功应用于测定 HCC 患者血清中 Mn、Fe、Cu、Zn、Ni、Se 的含量。所得结果与扇形场 ICP-MS (SF-ICP-MS) 的结果一致。与健康对照组相比，HCC 患者血清中的 Fe 和 Cu 含量较高，而 Zn 和 Se 含量较低。此外，HCC 患者和健康对照组血清中 Mn 和 Ni 的含量无显著差异。
