Mathur S, Chao L, Goust J M, Milroy G T, Woodley-Miller C, Caldwell J Z, Daru J, Williamson H O
Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425.
Am J Reprod Immunol Microbiol. 1988 May;17(1):5-13. doi: 10.1111/j.1600-0897.1988.tb00194.x.
Sera from three fertile men and four infertile men without sperm antibodies, 17 infertile men with sperm antibodies in serum and seminal plasma (S.P.), and 25 infertile men with sperm antibodies in S.P. were tested by Western Blot analysis against sperm membrane extracts and S.P. from fertile nonautoimmune men and infertile autoimmune men. Sera from fertile men reacted against common antigens with molecular weights (MW) of 28, 38, 48, 60, and 68 kD present on sperm from autoimmune and nonautoimmune men and special antigen of MW 76 kD on the sperm of fertile men. Sera from 15 of 17 (88%) autoimmune infertile men with sperm antibodies in serum and S.P. detected special antigens with MW of 58 kD (sera reactivity in 47% of these men), 43kD (in 29%), 30 kD (in 24%), 35 kD (in 18%), 52 kD (in 12%), 41 kD (in 6%), and 71 kD (in 6%) on the sperm of autoimmune men in addition to the common antigens. Sera from 15 of 25 (60%) men with sperm antibodies in their S.P. showed reactivity to special antigens with MW 52 kD (in 20%), 35 kD (in 16%), 41 kD (in 16%), 58 kD (in 8%), 70/71 kD (in 8%), 30 kD (in 8%), and 56 kD (in 4%). Sera from 18 of 42 (43%) infertile men with sperm antibodies also detected special antigens of MW 26, 46, and 76 kD present only in fertile men's sperm. Sera from only 15 of 42 (36%) autoimmune infertile men reacted against special antigens with MW 17, 20, 23, 30, 43, and 58 kD in the seminal plasma of autoimmune infertile men.(ABSTRACT TRUNCATED AT 250 WORDS)
采用蛋白质印迹分析，以可育非自身免疫男性和不育自身免疫男性的精子膜提取物及精浆为抗原，检测了3名可育男性、4名血清中无精子抗体的不育男性、17名血清和精浆中均有精子抗体的不育男性以及25名精浆中有精子抗体的不育男性的血清。可育男性血清可与自身免疫和非自身免疫男性精子上分子量（MW）为28、38、48、60和68kD的共同抗原以及可育男性精子上分子量为76kD的特殊抗原发生反应。17名血清和精浆中有精子抗体的自身免疫不育男性中，有15名（88%）的血清除了能检测到共同抗原外，还能检测到自身免疫男性精子上分子量为58kD（47%的男性血清有反应）、43kD（29%）、30kD（24%）、35kD（18%）、52kD（12%）、41kD（6%）和71kD（6%）的特殊抗原。25名精浆中有精子抗体的男性中，有15名（60%）的血清对分子量为52kD（20%）、35kD（16%）、41kD（16%）、58kD（8%）、70/71kD（8%）、30kD（8%）和56kD（4%）的特殊抗原呈反应性。42名有精子抗体的不育男性中，有18名（43%）的血清还检测到仅存在于可育男性精子中的分子量为26、46和76kD的特殊抗原。42名自身免疫不育男性中，只有15名（36%）的血清对自身免疫不育男性精浆中分子量为17、20、23、30、43和58kD的特殊抗原呈反应性。（摘要截取自250字）
