Congleton L, Potts W, Mathur S
Department of Urology, Medical University of South Carolina, Charleston.
Fertil Steril. 1989 Jul;52(1):106-12. doi: 10.1016/s0015-0282(16)60798-7.
Young adult male CD-1 mice were given intraperitoneal injections (IP) of saline (controls) and pooled sperm or seminal plasma of two autoimmune infertile men and two nonautoimmune fertile men (n = 40 per treatment). Other mice received only an oral challenge with the same antigens (oral controls; n = 20 per treatment). Three weeks after the booster challenge (day 36), 20 mice in each group were orally immunized with the antigens, whereas the other 20 were not (IP controls). Cytotoxic antibody titers (immunoglobulin M) to human sperm were significantly higher in mice IP immunized with sperm or seminal plasma from autoimmune infertile men or orally immunized with autoimmune men's sperm, in contrast to the controls. Oral challenge with sperm or seminal plasma of autoimmune infertile men after the IP immunization with the same resulted in significantly decreased cytotoxic sperm antibody titers (P less than 0.001 versus oral or IP controls in sperm immunization; P less than 0.001 versus IP controls in seminal plasma immunization). Fertility was unaffected by any mode of immunization. It is concluded that, in mice, sperm and seminal plasma antigens from autoimmune infertile men are more immunogenic than those from nonautoimmune fertile men, and oral challenge with the former after an IP establishment of cytotoxic sperm immunity desensitizes the immune mice. These findings may have practical implications in the diagnosis and immunotherapy of infertile men with cytotoxic sperm antibodies.
将成年雄性CD - 1小鼠腹腔注射（IP）生理盐水（对照组）以及两名自身免疫性不育男性和两名非自身免疫性可育男性的混合精子或精浆（每种处理n = 40）。其他小鼠仅口服相同抗原进行激发（口服对照组；每种处理n = 20）。在加强激发后三周（第36天），每组20只小鼠口服抗原进行免疫，而另外20只不进行（IP对照组）。与对照组相比，用自身免疫性不育男性的精子或精浆进行腹腔免疫或用自身免疫性男性的精子进行口服免疫的小鼠，其针对人精子的细胞毒性抗体滴度（免疫球蛋白M）显著更高。在用自身免疫性不育男性的精子或精浆进行腹腔免疫后，再口服相同物质进行激发，导致细胞毒性精子抗体滴度显著降低（与精子免疫中的口服或IP对照组相比，P < 0.001；与精浆免疫中的IP对照组相比，P < 0.001）。生育能力不受任何免疫方式的影响。结论是，在小鼠中，自身免疫性不育男性的精子和精浆抗原比非自身免疫性可育男性的更具免疫原性，并且在通过腹腔注射建立细胞毒性精子免疫后，用前者进行口服激发可使免疫小鼠脱敏。这些发现可能对具有细胞毒性精子抗体的不育男性的诊断和免疫治疗具有实际意义。
