Fodoulian Leon, Tuberosa Joël, Rossier Daniel, Boillat Madlaina, Kan Chenda, Pauli Véronique, Egervari Kristof, Lobrinus Johannes A, Landis Basile N, Carleton Alan, Rodriguez Ivan
Department of Genetics and Evolution, Faculty of Sciences, University of Geneva, quai Ernest-Ansermet 30, 1211 Geneva, Switzerland.
Department of Basic Neurosciences, Faculty of Medicine, University of Geneva, 1 rue Michel-Servet, 1211 Geneva, Switzerland.
iScience. 2020 Dec 18;23(12):101839. doi: 10.1016/j.isci.2020.101839. Epub 2020 Nov 25.
Reports indicate an association between COVID-19 and anosmia, as well as the presence of SARS-CoV-2 virions in the olfactory bulb. To test whether the olfactory neuroepithelium may represent a target of the virus, we generated RNA-seq libraries from human olfactory neuroepithelia, in which we found substantial expression of the genes coding for the virus receptor angiotensin-converting enzyme-2 (ACE2) and for the virus internalization enhancer TMPRSS2. We analyzed a human olfactory single-cell RNA-seq dataset and determined that sustentacular cells, which maintain the integrity of olfactory sensory neurons, express and . ACE2 protein was highly expressed in a subset of sustentacular cells in human and mouse olfactory tissues. Finally, we found transcripts in specific brain cell types, both in mice and humans. Sustentacular cells thus represent a potential entry door for SARS-CoV-2 in a neuronal sensory system that is in direct connection with the brain.
报告表明，新型冠状病毒肺炎（COVID-19）与嗅觉丧失之间存在关联，同时嗅球中也存在严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒粒子。为了测试嗅觉神经上皮是否可能是该病毒的一个靶点，我们从人类嗅觉神经上皮中生成了RNA测序文库，在其中我们发现编码病毒受体血管紧张素转换酶2（ACE2）和病毒内化增强剂跨膜丝氨酸蛋白酶2（TMPRSS2）的基因有大量表达。我们分析了一个人类嗅觉单细胞RNA测序数据集，并确定维持嗅觉感觉神经元完整性的支持细胞表达 和 。ACE2蛋白在人类和小鼠嗅觉组织的一部分支持细胞中高度表达。最后，我们在小鼠和人类的特定脑细胞类型中发现了 转录本。因此，在与大脑直接相连的神经元感觉系统中，支持细胞代表了SARS-CoV-2的一个潜在入口。
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