Systems Biotechnology, Technical University of Munich, Germany.
Bioresour Technol. 2021 Jan;319:124169. doi: 10.1016/j.biortech.2020.124169. Epub 2020 Sep 28.
In this work, the hydrogen-oxidizing bacterium Cupriavidus necator H16 was engineered for trehalose production from gaseous substrates. First, it could be shown that C. necator is a natural producer of trehalose when stressed with sodium chloride. Bioinformatic investigations revealed a so far unknown mode of trehalose and glycogen metabolism in this organism. Next, it was found that expression of the sugar efflux transporter A (setA) from Escherichia coli lead to a trehalose leaky phenotype of C. necator. Finally, the strain was characterized under autotrophic conditions using a H/CO/O-mixture and other substrates reaching titers of up to 0.47 g L and yields of around 0.1 g g. Taken together, this process represents a new way to produce sugars with high areal efficiency. With further metabolic engineering, an application of this technology for the renewable production of trehalose and other sugars, as well as for the synthesis of C-labeled sugars seems promising.
在这项工作中，对氢氧化细菌恶臭假单胞菌 H16 进行了工程改造，使其能够从气态底物生产海藻糖。首先，当用氯化钠胁迫时，可以证明恶臭假单胞菌是海藻糖的天然生产者。生物信息学研究揭示了该生物中一种迄今为止未知的海藻糖和糖原代谢方式。接下来，发现表达来自大肠杆菌的糖外排转运蛋白 A(setA) 导致恶臭假单胞菌出现海藻糖渗漏表型。最后，在使用 H/CO/O 混合物和其他底物的自养条件下对该菌株进行了表征，达到了高达 0.47 g L 的浓度和约 0.1 g g 的产率。总的来说，这个过程代表了一种具有高面积效率生产糖的新方法。通过进一步的代谢工程，该技术有望用于可再生生产海藻糖和其他糖，以及用于合成 C 标记的糖。
