Cheng Dengmiao, Liu Yuanwang, Shehata Ebrahim, Feng Yao, Lin Hui, Xue Jianming, Li Zhaojun
Research Center for Eco-Environmental Engineering, Dongguan University of Technology, Dongguan, 523808, China.
Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
J Hazard Mater. 2021 Jan 15;402:123710. doi: 10.1016/j.jhazmat.2020.123710. Epub 2020 Aug 22.
The dynamics of oxytetracycline (OTC), sulfamerazine (SM1), ciprofloxacin (CIP) and related antibiotic resistance genes (ARGs) during swine manure composting were compared between manure collected from swine fed a diet containing these three antibiotics (T) and manure directly spiked with these drugs (T). The composting removal efficiency of OTC (94.9 %) and CIP (87.8 %) in the T treatment was significantly higher than that of OTC (83.8 %, P < 0.01) and CIP (83.9 %, P < 0.05) in the T treatment, while SM1 exhibited no significant difference (P > 0.05) between the two treatments. Composting effectively reduced the majority of ARGs in both T and T types of manure, especially tetracycline resistance genes (TRGs). Compared with the T treatment, the abundance of some ARGs, such as tetG, qepA, sul1 and sul2, increased dramatically up to 309-fold in the T treatment. The microbial composition of the composting system changed significantly during composting due to antibiotic feeding. Redundancy analysis suggested that the abundance of ARGs had a considerable impact on alterations in the physicochemical parameters (C/N, pH and temperature) and bacterial communities (Actinobacteria, Proteobacteria and Firmicutes) during the composting of swine manure.
比较了从饲喂含这三种抗生素日粮的猪收集的粪便（T）和直接添加这些药物的粪便（T）在猪粪堆肥过程中土霉素（OTC）、磺胺甲基嘧啶（SM1）、环丙沙星（CIP）及相关抗生素抗性基因（ARGs）的动态变化。T处理中OTC（94.9%）和CIP（87.8%）的堆肥去除效率显著高于T处理中OTC（83.8%，P＜0.01）和CIP（83.9%，P＜0.05）的去除效率，而SM1在两种处理间无显著差异（P＞0.05）。堆肥有效降低了T和T两种类型粪便中的大多数ARGs，尤其是四环素抗性基因（TRGs）。与T处理相比，T处理中一些ARGs，如tetG、qepA、sul1和sul2的丰度急剧增加高达309倍。由于抗生素投喂，堆肥系统的微生物组成在堆肥过程中发生了显著变化。冗余分析表明，ARGs的丰度对猪粪堆肥过程中的理化参数（C/N、pH和温度）及细菌群落（放线菌、变形菌和厚壁菌）的变化有相当大的影响。
