Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture, China-New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China; Institute of Environmental Science and Research Ltd, Christchurch, 8041, New Zealand.
Research Center for Eco-Environmental Engineering, Dongguan University of Technology, Dongguan, 523808, China.
J Hazard Mater. 2020 May 5;389:122082. doi: 10.1016/j.jhazmat.2020.122082. Epub 2020 Jan 23.
Animal manure containing veterinary antibiotics is a significant source of microbial antibiotic resistance genes (ARGs). Composting of animal manure with wheat straw and sawdust was explored as a means to reduce ARGs load in the final material. The effects of ciprofloxacin, oxytetracycline, sulfamerazine on the bacterial community composition, and how this then affected the removal of seven tetracycline resistance genes (TARGs), four sulfonamide resistance genes (SARGs), and two fluoroquinolone resistance genes (QARGs) were investigated. Treatments receiving either ciprofloxacin or the three mixed antibiotics had reduced bacterial alpha-diversity and displayed shifts in the abundance of Proteobacteria and Firmicutes. This demonstrated that different antibiotics played an important role in bacterial community composition. Furthermore, variation in the physicochemical properties of compost, particularly pH and temperature, was also strongly linked to shifts in bacterial composition over time. Based on network analysis, the reduction of TARGs were associated with loss of Pseudomonas, Pseudoxanthomonas, Pusillimonas, Aquamicrobium, Ureibacillus, Lysinibacillus, Bacillus and Brachybacterium during the thermophilic stage. However, QARGs and SARGs were more strongly affected by the presence of multiple antibiotics. Our results have important implications for reducing the spread of certain ARGs by controlling the composting temperature, pH or the antibiotics species used in husbandry.
动物粪便中含有兽医抗生素，是微生物抗生素抗性基因（ARGs）的重要来源。本研究探索了用小麦秸秆和木屑对动物粪便进行堆肥，以减少最终物料中 ARGs 负荷。研究了环丙沙星、土霉素和磺胺甲恶唑对细菌群落组成的影响，以及这如何影响七种四环素抗性基因（TARGs）、四种磺胺类抗性基因（SARGs）和两种氟喹诺酮类抗性基因（QARGs）的去除。接受环丙沙星或三种混合抗生素处理的组，其细菌 α 多样性降低，变形菌门和厚壁菌门的丰度发生变化。这表明不同的抗生素在细菌群落组成中起着重要作用。此外，堆肥物理化学性质的变化，特别是 pH 值和温度，也与细菌组成随时间的变化密切相关。基于网络分析，在高温阶段，TARGs 的减少与假单胞菌、假单胞菌、普氏菌、微杆菌、乌尔西杆菌、短小杆菌、芽孢杆菌和短杆菌的减少有关。然而，QARGs 和 SARGs 受到多种抗生素存在的影响更大。我们的研究结果对通过控制堆肥温度、pH 值或畜牧业中使用的抗生素种类来减少某些 ARGs 的传播具有重要意义。
