Istituto Italiano di Tecnologia, Nanobiointeractions&Nanodiagnostics, Via Morego 30, 16163 Genova, Italy.
Department of Earth and Environmental Sciences (DISAT), University of Milano-Bicocca, P.za Della Scienza 1, 20126 Milan, Italy.
Biosensors (Basel). 2020 Nov 24;10(12):190. doi: 10.3390/bios10120190.
A colorimetric assay, exploiting the combination of loop-mediated isothermal amplification (LAMP) with DNA barcoding, was developed to address the authentication of some cephalopod species, a relevant group in the context of seafood traceability, due to the intensive processing from the fishing sites to the shelf. The discriminating strategy relies on accurate design of species-specific LAMP primers within the conventional 5' end of the mitochondrial COI DNA barcode region and allows for the identification of among two closely related and less valuable species. The assay, coupled to rapid genomic DNA extraction, is suitable for large-scale screenings and on-site applications due to its easy procedures, with fast (30 min) and visual readout.
一种比色分析检测方法，利用环介导等温扩增 (LAMP) 与 DNA 条码技术的结合，开发出来用于解决一些头足类物种的鉴定问题。在海产品可追溯性的背景下，这些物种非常重要，因为它们在从捕捞地到货架的过程中需要进行密集的加工。该鉴别策略依赖于在传统的线粒体 COI DNA 条码区域的 5' 端内对物种特异性 LAMP 引物进行精确设计，可用于两种密切相关但价值较低的物种的鉴定。该检测方法与快速基因组 DNA 提取相结合，由于其操作简单、快速（30 分钟）且具有可视化结果，适用于大规模筛查和现场应用。
