Velasco Amaya, Ramilo-Fernández Graciela, Sotelo Carmen G
Instituto de Investigaciones Marinas (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo (Pontevedra), Spain.
Foods. 2020 Mar 4;9(3):286. doi: 10.3390/foods9030286.
Cephalopods are very relevant food resources. The common cuttlefish () is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct ( < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates from other species of the genus and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products.
头足类动物是非常重要的食物资源。普通乌贼（）深受消费者喜爱，但在食品中缺乏快速鉴定它的方法。我们引入了一种新的小沟结合（MGB）TaqMan实时聚合酶链反应（PCR）方法来鉴定食品中的，以扩增线粒体细胞色素氧化酶I（COI）区域的122个碱基对（bp）片段。普通乌贼的参考样品和商业样品的阈值循环（Ct）平均值为14.40，而所检测的其他物种均未扩增，或显示出显著不同的Ct值（<0.001）。该系统计算出的效率为101%，检测到的最低DNA量为10 ng。未检测到与任何其他物种的交叉反应，因此，所设计的方法能够区分普通乌贼与乌贼属的其他物种以及其他头足类物种，并且适用于普通乌贼属的新鲜、冷冻、烤制、煮熟和罐装样品。该方法已被证明是可靠且快速的，可能成为控制乌贼产品欺诈行为的有用工具。
