Fan Yanmiao, Namata Faridah, Erlandsson Johan, Zhang Yuning, Wågberg Lars, Malkoch Michael
KTH Royal Institute of Technology, School of Chemical Science and Engineering, Fiber and Polymer Technology, Teknikringen 56-58, SE-100 44 Stockholm, Sweden.
KTH Royal Institute of Technology, Wallenberg Wood Science center. (WWSC), Teknikringen 56-58, SE-100 44 Stockholm, Sweden.
Pharmaceutics. 2020 Nov 25;12(12):1139. doi: 10.3390/pharmaceutics12121139.
Cationic dendrimers are intriguing materials that can be used as antibacterial materials; however, they display significant cytotoxicity towards diverse cell lines at high generations or high doses, which limits their applications in biomedical fields. In order to decrease the cytotoxicity, a series of biocompatible hybrid hydrogels based on cationic dendrimers and carboxylated cellulose nanofibrils were easily synthesized by non-covalent self-assembly under physiological conditions without external stimuli. The cationic dendrimers from generation 2 (G2) to generation 4 (G4) based on trimethylolpronane (TMP) and 2,2-bis (methylol)propionic acid (bis-MPA) were synthesized through fluoride promoted esterification chemistry (FPE chemistry). FTIR was used to show the presence of the cationic dendrimers within the hybrid hydrogels, and the distribution of the cationic dendrimers was even verified using elemental analysis of nitrogen content. The hybrid hydrogels formed from G3 and G4 showed 100% killing efficiency towards (), () and () with bacterial concentrations ranging from 10 CFU/mL to 10 CFU/mL. Remarkably, the hybrid hydrogels also showed good biocompatibility most probably due to the incorporation of the biocompatible CNFs that slowed down the release of the cationic dendrimers from the hybrid hydrogels, hence showing great promise as an antibacterial material for biomedical applications.
阳离子树枝状大分子是一种可作为抗菌材料的有趣材料；然而，它们在高代数或高剂量时对多种细胞系表现出显著的细胞毒性，这限制了它们在生物医学领域的应用。为了降低细胞毒性，在生理条件下，无需外部刺激，通过非共价自组装轻松合成了一系列基于阳离子树枝状大分子和羧化纤维素纳米纤维的生物相容性杂化水凝胶。基于三羟甲基丙烷（TMP）和2,2-双（羟甲基）丙酸（双-MPA）的第2代（G2）至第4代（G4）阳离子树枝状大分子通过氟化物促进的酯化化学（FPE化学）合成。傅里叶变换红外光谱（FTIR）用于显示杂化水凝胶中阳离子树枝状大分子的存在，并且使用氮含量的元素分析验证了阳离子树枝状大分子的分布。由G3和G4形成的杂化水凝胶对浓度范围为10 CFU/mL至10 CFU/mL的（）、（）和（）显示出100%的杀灭效率。值得注意的是，杂化水凝胶还表现出良好的生物相容性，这很可能是由于掺入了生物相容性的纤维素纳米纤维（CNFs），减缓了阳离子树枝状大分子从杂化水凝胶中的释放，因此作为生物医学应用的抗菌材料显示出巨大的前景。
