Liu Peng, Jonkheijm Pascal, Terstappen Leon W M M, Stevens Michiel
Department of Medical Cell BioPhysics, University of Twente, 7522 NB Enschede, The Netherlnds.
Department of Molecular Nanofabrication, University of Twente, 7522 NB Enschede, The Netherlands.
Cancers (Basel). 2020 Nov 26;12(12):3525. doi: 10.3390/cancers12123525.
Here, we review the characteristics and synthesis of magnetic nanoparticles (MNPs) and place these in the context of their usage in the immunomagnetic enrichment of Circulating Tumor Cells (CTCs). The importance of the different characteristics is explained, the need for a very specific enrichment is emphasized and different (commercial) magnetic separation techniques are shown. As the specificity of an MNP is in a large part dependent on the antibody coated onto the particle, different strategies in the coupling of specific antibodies as well as an overview of the available antibodies is given.
在此，我们回顾了磁性纳米颗粒（MNPs）的特性与合成，并将其置于循环肿瘤细胞（CTCs）免疫磁富集的应用背景中进行探讨。文中解释了不同特性的重要性，强调了实现高度特异性富集的必要性，并展示了不同的（商业）磁分离技术。由于磁性纳米颗粒的特异性在很大程度上取决于包被在颗粒上的抗体，因此本文介绍了偶联特异性抗体的不同策略以及可用抗体的概述。
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