Barthel Dana, Schuler Hannes, Galli Jonas, Borruso Luigimaria, Geier Jacob, Heer Katrin, Burckhardt Daniel, Janik Katrin
Laimburg Research Centre, Laimburg 6, Pfatten (Vadena), IT-39040 Auer (Ora), Italy.
Faculty of Science and Technology, Free University of Bozen-Bolzano, IT-39100 Bozen (Bolzano), Italy.
Insects. 2020 Nov 26;11(12):835. doi: 10.3390/insects11120835.
Apple proliferation is an economically important disease and a threat for commercial apple cultivation. The causative pathogen, the bacterium ' Phytoplasma mali', is mainly transmitted by , a phloem-feeding insect that develops on the apple tree ( spp.). To investigate the feeding behavior of adults of the phytoplasma vector in more detail, we used deep sequencing technology to identify plant-specific DNA ingested by the insect. Adult psyllids were collected in different apple orchards in the Trentino-South Tyrol region of northern Italy. DNA from the whole body of the insect was extracted and analyzed for the presence of plant DNA by performing PCR with two plant-specific primers that target the chloroplast regions and . DNA from 23 plant genera () and four plant families () of woody and herbaceous plant taxa was detected. Up to six and three plant genera and families, respectively, could be determined in single specimens. The results of this study contribute to a better understanding of the feeding behavior of adult .
苹果增殖病是一种具有重要经济影响的病害，对商业苹果种植构成威胁。致病病原体，即“苹果植原体”细菌，主要由一种以韧皮部为食的昆虫传播，这种昆虫在苹果树上发育（苹果属植物）。为了更详细地研究植原体传播媒介成虫的取食行为，我们使用深度测序技术来鉴定昆虫摄取的植物特异性DNA。在意大利北部特伦蒂诺-南蒂罗尔地区的不同苹果园中采集了成年木虱。提取昆虫全身的DNA，并通过使用针对叶绿体区域和的两种植物特异性引物进行PCR，分析植物DNA的存在情况。检测到来自23个植物属（）以及4个木本和草本植物类群的植物科（）的DNA。在单个样本中最多可分别确定6个植物属和3个植物科。这项研究的结果有助于更好地了解成年木虱的取食行为。
