一种用于检测侵袭性曲霉病患者半乳呋喃的新型灵敏夹心酶联免疫吸附测定法。
A new sensitive sandwich enzyme-linked immunosorbent assay to detect galactofuran in patients with invasive aspergillosis.
作者信息
Stynen D, Goris A, Sarfati J, Latgé J P
机构信息
Sanofi Diagnostics Pasteur, Genk, Belgium.
出版信息
J Clin Microbiol. 1995 Feb;33(2):497-500. doi: 10.1128/jcm.33.2.497-500.1995.
Abstract
A double-direct sandwich enzyme-linked immunosorbent assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoassay, which detects less than 1 ng of galactomannan per ml, was assessed in a retrospective study with samples from patients with invasive aspergillosis. Serum is more appropriate than urine for use in the search for circulating galactomannan. Antigenemia does not have a transient character. Galactomannan can be detected at least 39 days before the death of the patients.
摘要
设计了一种双直接夹心酶联免疫吸附测定法，该方法使用大鼠抗半乳甘露聚糖单克隆抗体作为受体和检测抗体。这种免疫测定法可检测每毫升低于1纳克的半乳甘露聚糖，在一项对侵袭性曲霉病患者样本的回顾性研究中进行了评估。在寻找循环半乳甘露聚糖时，血清比尿液更合适。抗原血症没有短暂性特征。在患者死亡前至少39天就能检测到半乳甘露聚糖。
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