Institut Für Mikro- Und Molekularbiologie, Justus-Liebig-Universität Giessen, IFZ, Giessen, Germany.
RNA Biol. 2021 Oct;18(10):1445-1457. doi: 10.1080/15476286.2020.1857520. Epub 2021 Jan 11.
Anoxygenic photosynthesis is an important pathway for to produce ATP under oxygen-limiting conditions. The expression of its photosynthesis genes is tightly regulated at transcriptional and post-transcriptional levels in response to light and oxygen signals, to avoid photooxidative stress by the simultaneous presence of pigments, light and oxygen. The operon encodes pigment-binding proteins of the light-harvesting complex I (genes and ), of the reaction centre (genes and ), a scaffold protein (gene ) and includes the gene for sRNA PcrX. Segmental differences in the stability of the mRNA contribute to the stoichiometry of LHI to RC complexes. With asPcrL we identified the third sRNA and the first antisense RNA that is involved in balancing photosynthesis gene expression in . asPcrL influences the stability of the mRNA but not of the mRNA and consequently the stoichiometry of photosynthetic complexes. By base pairing to the region asPcrL promotes RNase III-dependent degradation of the mRNA. Since asPcrL is activated by the same protein regulators as the operon including PcrX it is part of an incoherent feed-forward loop that fine-tunes photosynthesis gene expression.[Figure: see text].
厌氧光合作用是 产生 ATP 的重要途径在氧气限制条件下。其光合作用基因的表达在转录和转录后水平受到严格调控，以响应光和氧信号，避免色素、光和氧同时存在所产生的光氧化应激。 操纵子编码光捕获复合物 I 的色素结合蛋白（基因 和 ）、反应中心（基因 和 ）、支架蛋白（基因 ），并包括 sRNA PcrX 的基因。 在 mRNA 的稳定性方面存在节段性差异有助于 LHI 到 RC 复合物的化学计量比。通过 asPcrL，我们鉴定了第三个 sRNA 和第一个反义 RNA，它们参与平衡 在 中的光合作用基因表达。asPcrL 影响 mRNA 的稳定性而不影响 mRNA，因此影响光合复合物的化学计量比。通过与 区域的碱基配对，asPcrL 促进 RNase III 依赖性降解 mRNA。由于 asPcrL 被 操纵子的相同蛋白调节剂激活，包括 PcrX，因此它是一个非相干前馈环的一部分，可微调光合作用基因表达。[图：见正文]。
