Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, College of Veterinary Medicine, Northeast Agricultural University , Harbin, P.R. China.
College of Veterinary Medicine, China Agricultural University , Beijing, P.R. China.
Virulence. 2021 Dec;12(1):20-34. doi: 10.1080/21505594.2020.1857572.
Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which significantly affects the production performance of cattle, causing serious economic losses to the cattle industries worldwide. Up to now, some mechanisms involved in host-BVDV interaction are still not fully understood. The discovery of long non-coding RNAs (lncRNAs) has provided a new perspective on gene regulation in diverse biological contexts, particularly in viral infection and host immune responses. However, little is known about the profiles and functions of lncRNAs in host cells in response to BVDV infection. Here, we utilized Illumina sequencing to explore lncRNAs profiles in cytopathic (CP) biotype BVDV-infected MDBK cells to further reveal the potential roles of lncRNAs in BVDV infection and host-BVDV interaction with integrated analysis of lncRNAs and mRNA expression profiles. A total of 1747 significantly differentially expressed genes, DEGs (156 lncRNAs and 1591 mRNAs) were obtained via RNA-seq in BVDV-infected MDBK cells compared to mock-infected cells. Next, these DE lncRNAs and mRNAs were subjected to construct lncRNAs-mRNAs co-expression network followed by the prediction of potential functions of the DE lncRNAs. Co-expression network analysis elucidated that DE lncRNAs were significant enrichment in NOD-like receptor, TNF, NF-ĸB, ErbB, Ras, apoptosis, and fatty acid biosynthesis pathways, indicating that DE lncRNAs play important roles in host-BVDV interactions. Our data give an overview of changes in transcriptome and potential roles of lncRNAs, providing molecular biology basis for further exploring the mechanisms of host-BVDV interaction.
牛病毒性腹泻病毒（BVDV）是牛病毒性腹泻-黏膜病的病原体，它严重影响牛的生产性能，给全球的牛业造成严重的经济损失。迄今为止，宿主与 BVDV 相互作用的一些机制仍未完全阐明。长非编码 RNA（lncRNA）的发现为不同生物背景下的基因调控提供了新视角，特别是在病毒感染和宿主免疫反应中。然而，宿主细胞中 lncRNA 对 BVDV 感染的反应及其功能仍知之甚少。在这里，我们利用 Illumina 测序技术来探索致细胞病变（CP）生物型 BVDV 感染的 MDBK 细胞中的 lncRNA 图谱，以进一步揭示 lncRNA 在 BVDV 感染和宿主- BVDV 相互作用中的潜在作用，并通过 lncRNA 和 mRNA 表达谱的综合分析来揭示 lncRNA 在 BVDV 感染和宿主- BVDV 相互作用中的潜在作用。与 mock 感染细胞相比，通过 RNA-seq 在 BVDV 感染的 MDBK 细胞中获得了 1747 个显著差异表达的基因（156 个 lncRNA 和 1591 个 mRNA）。接下来，这些差异表达的 lncRNA 和 mRNA 被用于构建 lncRNA-mRNA 共表达网络，然后预测潜在的 DE lncRNA 功能。共表达网络分析表明，DE lncRNA 显著富集在 NOD 样受体、TNF、NF-κB、ErbB、Ras、凋亡和脂肪酸生物合成途径中，表明 DE lncRNA 在宿主- BVDV 相互作用中发挥重要作用。我们的数据概述了转录组的变化和 lncRNA 的潜在作用，为进一步探索宿主- BVDV 相互作用的机制提供了分子生物学基础。
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