印度东北部猪群中牛病毒性腹泻病毒的证据——基因分析与特征描述
Evidence of BVDV in Pigs from North Eastern Part of India- Genetic Profiling and Characterisation.
作者信息
Chakraborty Amit Kr, Mukherjee Priyanka, Karam Amarjit, Das Samir, Barkalita Luit, Puro Kekungo, Sanjukta Rajkumari, Ghatak Sandeep, Sakuntala Ingudam, Laha Ram Gopal, Borah Prabodh, Ngachan S V, Sharma Indu, Sen Arnab
机构信息
Division of Animal Health, ICAR Research Complex for NEH, Barapani - 793103, India.
Department of Microbiology, Assam University, Silchar - 788011, India.
出版信息
Open Virol J. 2018 Aug 31;12:110-120. doi: 10.2174/1874357901812010110. eCollection 2018.
INTRODUCTION
The work has been attempted to detect and genetically characterise the nature of Bovine Viral Diarrhea Virus (BVDV) isolates from the porcine population of the north east.
METHODS AND MATERIAL
The samples have been collected over a two year period and are from areas where there is a mixed and integrated rearing of livestock in close proximity. The isolates were identified, cloned and sequenced using BVD specific genomic primers for two important domains viz., E-2 and 5' UTR.
RESULTS
Porcine BVD Sequences were analysed phylogenetically. Divergence in 3 sequences is noted in the 5' UTR region that are forming a clear outlier group while E-2 sequences are coming close to BVDV group but forming a separate cluster.
引言
本研究旨在检测来自东北地区猪群的牛病毒性腹泻病毒（BVDV）分离株，并对其进行基因特征分析。
方法与材料
样本采集历时两年，来自家畜混合养殖且距离较近的地区。使用针对两个重要区域即E-2和5'非翻译区（UTR）的BVD特异性基因组引物对分离株进行鉴定、克隆和测序。
结果
对猪BVD序列进行了系统发育分析。在5'UTR区域发现3个序列存在差异，形成一个明显偏离的群体，而E-2序列接近BVDV群体，但形成一个单独的簇。
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