Krylov V N, Karapetian A T
Genetika. 1977;13(6):1079-88.
The adsorption of 23 new lambdoid bacteriophages to 547 strains which isolated from natural population of Enterobacteriaceae was studied. The frequency of positive combinations of phage-bacterium with adsorption is not more than 2%. A study of possible causes of limited growth of lambdoid phages in the bacterial strains revealed that neither homoimmune prophage nor prophage P2 are single factors of the growth limitation. It is found that in natural populations a selection of bacterial strains with the least limitation of phage takes place. Three cases of killing bacteria after infection with high multiplicity are found. The reason of the killing effect is manifestation of some functions by infecting phages. A new restriction-modification system is found which differs from restriction-modification system A, B, K, 15, P1, EcoRI, EcoRII. The most of strains, which adsorb phages but do not support their growth, are supposed to possess several mechanisms of restriction. Thus, the search of new restriction system in Escherichia coli is worthwhile.
研究了23种新型λ样噬菌体对从肠杆菌科自然群体中分离出的547株菌株的吸附情况。噬菌体与细菌吸附阳性组合的频率不超过2%。对λ样噬菌体在细菌菌株中生长受限的可能原因进行的研究表明，同免疫原噬菌体和P2原噬菌体都不是生长受限的单一因素。研究发现，在自然群体中会发生对噬菌体限制作用最小的细菌菌株的选择。发现了3例高感染复数感染后细菌被杀死的情况。杀伤作用的原因是感染噬菌体表现出了某些功能。发现了一种新的限制修饰系统，它不同于A、B、K、15、P1、EcoRI、EcoRII限制修饰系统。大多数吸附噬菌体但不支持其生长的菌株被认为具有多种限制机制。因此，在大肠杆菌中寻找新的限制系统是值得的。
