Upmal M R, Kobets N S, Il'ina T S, Piruzian E S
Genetika. 1984 Jun;20(6):924-32.
Properties of mud mutations affecting intracellular development of bacteriophage Mu have been studied. We demonstrate pleiotropy of these mutations as well as their effect on the development of Mu-like heteroimmune D108 phage and P1 phage, on the ability to inherit Mu prophage within the chromosome during conjugation, and the abortive inheritance of chromosomal markers during P1 transduction. Experimental evidence is given to support the concept that the effect of the mutations has no relation to the mutants' impaired ability to adsorb the phages. Genetic analysis which involved conjugational crossings using different Hfr donors and F' plasmids made it possible to localize the mud-1 mutation between ilv and metE genes in the region of 84-85 min of the Escherichia coli K-12 genetic map. The nature of mutations and a possible functional role of the mud-impaired gene is discussed.
对影响噬菌体Mu细胞内发育的泥型突变的特性进行了研究。我们证明了这些突变的多效性，以及它们对Mu样异免疫D108噬菌体和P1噬菌体发育的影响，对在接合过程中染色体上Mu原噬菌体遗传能力的影响，以及在P1转导过程中染色体标记的流产遗传。提供了实验证据来支持这样的概念，即突变的影响与突变体吸附噬菌体能力受损无关。使用不同的Hfr供体和F'质粒进行接合杂交的遗传分析使得将mud-1突变定位在大肠杆菌K-12遗传图谱84-85分钟区域的ilv和metE基因之间成为可能。讨论了突变的性质以及mud受损基因可能的功能作用。
