[紫外线损伤噬菌体的修复]。

[Reparation of UV-damaged bacteriophage ed].

作者信息

Preobrazhenskaia E S, Lisovskaia K V, Kriviskiĭ A S

出版信息

Genetika. 1977;13(6):1108-18.

Abstract

A repair of UV-damaged phage DNA in the "phage-host" system in accordance with the excision reparative mechanism is demonstrated by means of centrifugation in alkaline sucrose gradient of virulent 3H-thymidine labelled phage sd. The increase of the transfectants quantity of UV-irradiated DNA on uvr+ bacteria compatibly to uvr- bacteria evidences that the bacterial host participates in phage reparation. Caffeine inhibition of UV-irradiated phage sd survival confirms the participation of cell-host in reparation of UV-damaged phage.

摘要

通过对强毒性3H-胸腺嘧啶标记的噬菌体sd在碱性蔗糖梯度中进行离心，证明了在“噬菌体-宿主”系统中，按照切除修复机制对紫外线损伤的噬菌体DNA进行修复。与uvr-细菌相比，uvr+细菌上紫外线照射DNA的转染子数量增加，这证明细菌宿主参与了噬菌体修复。咖啡因对紫外线照射的噬菌体sd存活的抑制作用证实了细胞宿主参与了紫外线损伤噬菌体的修复。

相似文献

1

[Reparation of UV-damaged bacteriophage ed].[紫外线损伤噬菌体的修复]。

Genetika. 1977;13(6):1108-18.

2

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3

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7

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