Hussain Saddam, Park Soo-Young
School of Applied Chemical Engineering, Polymeric Nano Materials Laboratory, Kyungpook National University, Daegu 41566, Republic of Korea.
ACS Sens. 2020 Dec 24;5(12):3988-3998. doi: 10.1021/acssensors.0c01757. Epub 2020 Dec 1.
A wearable noninvasive biosensor for in situ urea detection and quantification was developed using a urease-immobilized photonic interpenetrating polymer network (IPN) film. The photonic IPN film was intertwined with solid-state cholesteric liquid crystals (CLC) and a poly(acrylic acid) (PAA) network on a flexible poly(ethylene terephthalate) substrate adhered to a poly(dimethylsiloxane) (PDMS) chip that was fabricated using an aluminum mold. The presence of urea in the chemical matrix of human sweat red-shifted the reflected color of the photonic IPN film, and quantification was achieved by observing the wavelength at the photonic band gap (λ) with a limit of detection of 0.4 mM and a linear range of 0.9-50 mM. The color changes observed in the photonic IPN film were digitalized using the CIE 1931 coordinates on a cell phone image, thereby enabling fast, direct diagnosis via a downloadable app. This novel PDMS chip can be expanded for use with other biosensors.
利用固定有脲酶的光子互穿聚合物网络（IPN）薄膜，开发了一种用于原位尿素检测和定量的可穿戴无创生物传感器。该光子IPN薄膜与固态胆甾型液晶（CLC）和聚（丙烯酸）（PAA）网络交织在一起，位于附着在聚二甲基硅氧烷（PDMS）芯片上的柔性聚对苯二甲酸乙二酯基底上，该PDMS芯片是使用铝模具制造的。人体汗液化学基质中尿素的存在使光子IPN薄膜的反射颜色发生红移，通过观察光子带隙（λ）处的波长实现定量，检测限为0.4 mM，线性范围为0.9 - 50 mM。利用手机图像上的CIE 1931坐标对光子IPN薄膜中观察到的颜色变化进行数字化处理，从而能够通过可下载的应用程序进行快速、直接的诊断。这种新型PDMS芯片可扩展用于其他生物传感器。
