State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, Department of General Dentistry & Emergency, the Hospital of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Center, the Hospital of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Int J Immunopathol Pharmacol. 2020 Jan-Dec;34:2058738420974893. doi: 10.1177/2058738420974893.
Periodontitis is an inflammatory disease caused by host immune response, resulting in a loss of periodontium and alveolar bone. Immune cells, such as T cells and macrophages, play a critical role in the periodontitis onset. Halofuginone, a natural quinazolinone alkaloid, has been shown to possess anti-fibrosis, anti-cancer, and immunomodulatory properties. However, the effect of halofuginone on periodontitis has never been reported. In this study, a ligature-induced mice model of periodontitis was applied to investigate the potential beneficial effect of halofuginone on periodontitis. We demonstrated that the administration of halofuginone significantly reduced the expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in vivo, and markedly suppressed immune cell infiltration into the infected sites. Furthermore, we also observed that halofuginone treatment blocked the T-helper 17 (Th17) cell differentiation in vivo and in vitro. We demonstrated for the first time that halofuginone alleviated the onset of periodontitis through reducing immune responses.
牙周炎是一种由宿主免疫反应引起的炎症性疾病，导致牙周组织和牙槽骨丧失。免疫细胞，如 T 细胞和巨噬细胞，在牙周炎的发病中起着关键作用。哈尔福林酮是一种天然的喹唑啉酮生物碱，具有抗纤维化、抗癌和免疫调节作用。然而，哈尔福林酮对牙周炎的影响从未有过报道。在本研究中，应用结扎诱导的牙周炎小鼠模型研究了哈尔福林酮对牙周炎的潜在有益作用。结果表明，哈尔福林酮的给药显著降低了体内促炎细胞因子（IL-1β、IL-6 和 TNF-α）的表达水平，并显著抑制了免疫细胞浸润到感染部位。此外，我们还观察到哈尔福林酮治疗阻断了体内和体外 Th17 细胞的分化。我们首次证明，哈尔福林酮通过减少免疫反应缓解了牙周炎的发病。
