胎盘间充质干细胞及其条件培养基对局部辐射损伤的有效性评估
Evaluation of the Effectiveness of Mesenchymal Stem Cells of the Placenta and Their Conditioned Medium in Local Radiation Injuries.
作者信息
Brunchukov Vitaliy, Astrelina Tatiana, Usupzhanova Daria, Rastorgueva Anna, Kobzeva Irina, Nikitina Victoria, Lishchuk Sergei, Dubova Elena, Pavlov Konstantin, Brumberg Valentin, Benderitter Marc, Samoylov Alexander
机构信息
State Research Center Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 123098 Moscow, Russia.
Institute for Radiological Protection and Nuclear Safety (IRSN), 92260 Fontenay-aux-Roses, France.
出版信息
Cells. 2020 Nov 29;9(12):2558. doi: 10.3390/cells9122558.
BACKGROUND
The search for an effective therapy for local radiation injuries (LRI) is urgent; one option is mesenchymal stem cells (MSC) derived from the placenta and their conditioned medium for the regenerative processes of the skin.
METHODS
We used 80 animals, randomly assigned to four groups: control (C) animals that did not receive therapy; control with the introduction of culture medium concentrate (CM); introduction of MSCs (PL); introduction of CMPL. LRI modeling was performed on an X-ray machine at a dose of 110 Gy. Histological and immunohistochemical tests were performed.
RESULTS
On the 112th day, the area of the open wound surface in the CMPL group was 6.7 times less than in the control group. Complete healing of the open wound surface of the skin in the CM group was observed in 40%, in CMPL 60%, in the PL group 20%, and in the C group there were no animals with a prolonged wound defect. A decrease in inflammatory processes was observed in the CMPL group.
CONCLUSIONS
the use of a concentrate of conditioned MSCs (CMPL group) in severe LRI in laboratory animals accelerates the transition of the wound process to the stage of regeneration and epithelization.
背景
迫切需要寻找一种治疗局部放射性损伤（LRI）的有效疗法；一种选择是源自胎盘的间充质干细胞（MSC）及其条件培养基用于皮肤的再生过程。
方法
我们使用了80只动物，随机分为四组：未接受治疗的对照组（C）动物；引入培养基浓缩物（CM）的对照组；引入间充质干细胞（PL）；引入CMPL。在X射线机上以110 Gy的剂量进行LRI建模。进行了组织学和免疫组织化学测试。
结果
在第112天，CMPL组的开放性伤口表面面积比对照组小6.7倍。CM组皮肤开放性伤口表面40%完全愈合，CMPL组60%，PL组20%，C组没有动物存在伤口缺陷延长的情况。CMPL组炎症过程有所减轻。
结论
在实验动物的严重LRI中使用条件性间充质干细胞浓缩物（CMPL组）可加速伤口过程向再生和上皮化阶段的转变。
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