Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan.
Division of Breast Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan.
Genes (Basel). 2020 Nov 28;11(12):1436. doi: 10.3390/genes11121436.
Hypersialylation caused by the overexpression of sialyltransferases (STs) is a common feature in cancer that is associated with several characteristics of tumorigenesis. Thus, identifying cancer-associated STs is critical for cancer therapy. However, ST screening has been frequently conducted in cell line models. In this study, we conducted a comprehensive analysis of STs in the clinical database and identified the STs related with the survival of breast cancer patients. RNA sequencing (RNA-Seq) data of 496 patients were obtained from The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA). Of the eight mapped STs, , and met the acceptable area under the curve (AUC) criteria for overall survival (OS). Using Kaplan-Meier methods, we determined that high expression of was associated with poor 10-year OS in all patients, triple-negative breast cancer (TNBC), and non-TNBC patients, and poor disease-free survival (DFS) rates particularly in TNBC. also had superior AUC values in terms of OS/DFS. High levels showed a higher risk for poor OS in different groups of patients and a higher risk for poor DFS particularly in TNBC. In summary, we conducted a comprehensive analysis of STs from the clinical database and identified as a crucial survival-related ST, which might be a potential therapeutic target for breast cancer and TNBC patients.
高唾液酸化是由于唾液酸转移酶（sialyltransferases，STs）的过度表达引起的，是癌症的一个常见特征，与肿瘤发生的几个特征有关。因此，鉴定与癌症相关的 STs 对于癌症治疗至关重要。然而，ST 筛选经常在细胞系模型中进行。在本研究中，我们对临床数据库中的 STs 进行了全面分析，并鉴定了与乳腺癌患者生存相关的 STs。从癌症基因组图谱乳腺浸润性癌（TCGA-BRCA）中获得了 496 名患者的 RNA 测序（RNA-Seq）数据。在 8 个映射的 STs 中， ，和 满足了总体生存（OS）的可接受的曲线下面积（AUC）标准。使用 Kaplan-Meier 方法，我们确定高表达 与所有患者、三阴性乳腺癌（TNBC）和非 TNBC 患者的 10 年 OS 不良以及 TNBC 患者的无病生存（DFS）率差有关。 在 OS/DFS 方面也具有较高的 AUC 值。高 水平在不同患者组中显示出 OS 不良的高风险，尤其是在 TNBC 中 DFS 不良的风险更高。总之，我们对临床数据库中的 STs 进行了全面分析，鉴定 为关键的生存相关 ST，它可能是乳腺癌和 TNBC 患者的潜在治疗靶点。
