Hu Xu, Yang Yanfei, Wang Yaohui, Ren Shangqing, Li Xiang
Institute of Urology, Department of Urology, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
The Third Xiangya Hospital of Central South Hospital, Changsha, China.
Front Pharmacol. 2022 Jul 7;13:901518. doi: 10.3389/fphar.2022.901518. eCollection 2022.
Clear-cell renal cell carcinoma (ccRCC) is one of the most common urological cancers. The tumor microenvironment plays an important role in tumor development. The present study was conducted to identify novel immune-related biomarkers. The differentially expressed genes were identified using the ESTIMATE algorithm base on GEO and TCGA databases. The Kaplan-Meier survival curve and univariate and multivariate analyses were performed. The association between ST8SIA1 and the immune system was explored. The gene set enrichment analysis (GSEA) and online databases were used for functional annotation. ST8SIA1 was identified as a potential prognostic gene. Elevated ST8SIA1 was observed in the tumor tissues compared with adjacent normal tissues and associated with higher T stage and advanced TNM stage (all < 0.05). The mRNA and protein levels of ST8SIA1 in cancer tissues and cells are also upregulated. The Kaplan-Meier survival curve and univariate and multivariate analyses showed that higher expression of ST8SIA1 was associated with worse OS (all < 0.05). ST8SIA1 expression levels were negatively correlated with tumor purity and positively associated with infiltrated immune cells and expression of immune checkpoint genes. Function analysis also revealed that ST8SIA1 was significantly associated with immune-related pathways. In conclusion, ST8SIA1 was identified as an immune-related gene and a potential target in ccRCC patients. Further relevant studies are required to validate our findings.
透明细胞肾细胞癌（ccRCC）是最常见的泌尿系统癌症之一。肿瘤微环境在肿瘤发展中起重要作用。本研究旨在鉴定新的免疫相关生物标志物。基于GEO和TCGA数据库，使用ESTIMATE算法鉴定差异表达基因。进行了Kaplan-Meier生存曲线以及单因素和多因素分析。探讨了ST8SIA1与免疫系统之间的关联。使用基因集富集分析（GSEA）和在线数据库进行功能注释。ST8SIA1被鉴定为一个潜在的预后基因。与相邻正常组织相比，在肿瘤组织中观察到ST8SIA1升高，并且与更高的T分期和晚期TNM分期相关（均P<0.05）。癌组织和细胞中ST8SIA1的mRNA和蛋白质水平也上调。Kaplan-Meier生存曲线以及单因素和多因素分析表明，ST8SIA1的高表达与较差的总生存期相关（均P<0.05）。ST8SIA1表达水平与肿瘤纯度呈负相关，与浸润免疫细胞和免疫检查点基因的表达呈正相关。功能分析还显示，ST8SIA1与免疫相关途径显著相关。总之，ST8SIA1被鉴定为ccRCC患者中的一个免疫相关基因和潜在靶点。需要进一步的相关研究来验证我们的发现。
