Sato Kiminori, Chitose Shun-Ichi, Sato Kiminobu, Sato Fumihiko, Ono Takeharu, Umeno Hirohito
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan.
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan.
Auris Nasus Larynx. 2021 Aug;48(4):704-709. doi: 10.1016/j.anl.2020.11.011. Epub 2020 Nov 28.
The human adult vocal fold mucosa, especially, superficial layer of the lamina propria (Reinke's space) has attracted notice as an important vibrating structure. However, fine structures of the stratified squamous epithelium of the human adult vocal fold, which is another histological component of the mucosa, remain enigmatic.
Three normal human adult vocal folds and epiglottises and three newborn vocal folds were investigated. Observations using transmission electron microscopy and light microscopy including immunohistochemistry were performed.
The most obvious feature of the epithelium of the human adult vocal folds was that the intercellular spaces between adjacent epithelial cells were large (984 ± 186 nm) compared with the stratified squamous epithelium of the human adult epiglottis and the human newborn vocal fold. Even though intercellular spaces between adjacent epithelial cells of the human adult vocal fold were large, desmosomes at the junctions of two adjacent epithelial cells made firm intercellular adhesion. Tonofilaments composed of the bundles of intermediate filaments anchored to the desmosomes. Desmosomes formed a continuous cytoskeletal network throughout the epithelial cells and epithelium of the human adult vocal fold. In addition, a great deal of E-cadherin (adhesive glycoprotein) was present between epithelial cells especially the lower half of the stratified squamous epithelium of the human adult vocal fold.
From the functional morphological point of view, stratified squamous epithelium of the human adult vocal fold seems to form a structural framework of tensile strength with pliability suitable structure for vibration.
人类成年声带黏膜，尤其是固有层浅层（Reinke间隙）作为重要的振动结构已受到关注。然而，成年人类声带复层鳞状上皮这一黏膜的另一组织学成分的精细结构仍不清楚。
研究了3例正常成年人类声带和会厌以及3例新生儿声带。采用透射电子显微镜和包括免疫组织化学在内的光学显微镜进行观察。
成年人类声带上皮最明显的特征是，与成年人类会厌和新生儿声带的复层鳞状上皮相比，相邻上皮细胞之间的细胞间隙较大（984±186nm）。尽管成年人类声带相邻上皮细胞之间的细胞间隙较大，但相邻两个上皮细胞连接处的桥粒使细胞间黏附牢固。由中间丝束组成的张力丝锚定在桥粒上。桥粒在成年人类声带的整个上皮细胞和上皮中形成连续的细胞骨架网络。此外，上皮细胞之间，尤其是成年人类声带复层鳞状上皮下半部分，存在大量E-钙黏蛋白（黏附糖蛋白）。
从功能形态学角度来看，成年人类声带的复层鳞状上皮似乎形成了一个具有拉伸强度且柔韧性适合振动的结构框架。
