Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
Mol Biol Rep. 2020 Dec;47(12):9459-9468. doi: 10.1007/s11033-020-06027-9. Epub 2020 Dec 1.
Ovarian cancer, the most lethal gynecological cancer, is the fifth most common cause of cancer-related deaths in women. A cost-effective and non-invasive early screening method for ovarian cancer is required to reduce the high mortality rate. Saliva is a clinically informative unique fluid, which is useful for novel approaches to prognosis, clinical diagnosis, and monitoring for non-invasive detection of disease. Multimerin1 (MMRN1) is a di-sulfide linked homo-polymeric glycoprotein from EMILIN family. Altered expression of MMRN1 has been reported in hepatocellular carcinoma, cervical cancer, and ovarian cancer. But, its role in epithelial ovarian cancer (EOC) is not clear and well documented. In this study, expression of Multimerin 1 was validated in saliva and tissues of EOC patients and age-matched controls by western blotting, ELISA, RT-PCR, and immunohistochemistry. Significant over expression of MMRN1 was observed by western blot and ELISA in saliva samples of EOC patients. The average concentration of MMRN1 in the saliva of healthy controls was 28.7 pg/ml (SE ± 1.76), 42.53 pg/ml (SE ± 4.06) in low grade and 52.91 pg/ml (SE ± 4.24) with p < 0.01 in high-grade EOC. Upregulated cytoplasmic expression of MMRN1 was observed in EOC tissue by immunohistochemistry. Our results suggest that MMRN1 expression is associated with EOC progression and MMRN1 may be potential biomarker candidates for early-stage EOC detection however further experiments are required in a large cohort to establish this proposition. Also, saliva can be explored as a novel medium for ovarian cancer diagnosis.
卵巢癌是致死率最高的妇科癌症，也是女性癌症相关死亡的第五大主要原因。因此，需要一种经济有效的、非侵入性的早期筛查方法来降低高死亡率。唾液是一种具有临床意义的独特液体，可用于通过新型方法来预测、临床诊断和监测疾病，以实现非侵入性检测。多聚蛋白 1（MMRN1）是一种来自 EMILIN 家族的二硫键连接的同型聚合糖蛋白。已有研究报道，MMRN1 在肝癌、宫颈癌和卵巢癌中的表达发生改变。但是，其在卵巢上皮性癌（EOC）中的作用尚不清楚，也没有相关的文献记载。在这项研究中，通过 Western blot、ELISA、RT-PCR 和免疫组化，在 EOC 患者的唾液和组织中验证了多聚蛋白 1 的表达。Western blot 和 ELISA 分析显示，EOC 患者唾液样本中 MMRN1 的表达显著上调。健康对照组唾液中 MMRN1 的平均浓度为 28.7pg/ml（SE ± 1.76），低级别组为 42.53pg/ml（SE ± 4.06），高级别组为 52.91pg/ml（SE ± 4.24），高级别组与低级别组和健康对照组相比差异均有统计学意义（p<0.01）。免疫组化分析显示，EOC 组织中细胞质 MMRN1 的表达上调。我们的研究结果表明，MMRN1 的表达与 EOC 的进展相关，MMRN1 可能是早期 EOC 检测的潜在生物标志物候选物，但需要在更大的队列中进行进一步的实验来验证这一假设。此外，唾液可以作为一种新型的卵巢癌诊断介质进行探索。
