Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi 110029, India.
Int J Mol Sci. 2023 Sep 18;24(18):14239. doi: 10.3390/ijms241814239.
The asymptomatic nature, high rate of disease recurrence, and resistance to platinum-based chemotherapy highlight the need to identify and characterize novel target molecules for ovarian cancer. Fibroblast growth factor 8 (FGF8) aids in the development and metastasis of ovarian cancer; however, its definite role is not clear. We employed ELISA and IHC to examine the expression of FGF8 in the saliva and tissue samples of epithelial ovarian cancer (EOC) patients and controls. Furthermore, various cell assays were conducted to determine how FGF8 silencing influences ovarian cancer cell survival, adhesion, migration, and invasion to learn more about the functions of FGF8. In saliva samples, from controls through low-grade to high-grade EOC, a stepped overexpression of FGF8 was observed. Similar expression trends were seen in tissue samples, both at protein and mRNA levels. FGF8 gene silencing in SKOV3 cells adversely affected various cell properties essential for cancer cell survival and metastasis. A substantial reduction was observed in the cell survival, cell adhesion to the extracellular matrix, migration, and adhesion properties of SKOV3 cells, suggesting that FGF8 plays a crucial role in the development of EOC. Conclusively, this study suggests a pro-metastatic function of FGF8 in EOC.
FGF8 促进卵巢癌的发展和转移；然而，其确切作用尚不清楚。我们采用 ELISA 和 IHC 检测上皮性卵巢癌（EOC）患者和对照者唾液和组织样本中 FGF8 的表达。此外，我们还进行了各种细胞实验，以确定 FGF8 沉默如何影响卵巢癌细胞的存活、黏附、迁移和侵袭，从而更深入地了解 FGF8 的功能。在唾液样本中，我们观察到从对照者到低级别再到高级别 EOC，FGF8 的表达呈逐步上调。在组织样本中，无论是在蛋白水平还是在 mRNA 水平，都观察到了相似的表达趋势。FGF8 基因沉默在 SKOV3 细胞中对各种对癌细胞存活和转移至关重要的细胞特性产生了不良影响。SKOV3 细胞的细胞存活、细胞与细胞外基质的黏附、迁移和黏附特性都显著减少，这表明 FGF8 在 EOC 的发生发展中发挥了重要作用。综上所述，本研究表明 FGF8 在 EOC 中具有促转移的功能。
