Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy.
Department of Experimental and Clinical Medicine, University Magna Græcia of Catanzaro, 88100 Catanzaro, Italy.
Int J Mol Sci. 2020 Nov 30;21(23):9113. doi: 10.3390/ijms21239113.
The prevalence of idiopathic male infertility is high, up to 75% of patients with abnormal sperm parameters. Hence, the research of its causes is mandatory. Oxidative stress (OS) can be responsible for male infertility in 30-80% of cases. In recent years, seminal plasma (SP) proteomics has developed as a useful tool to provide biomarkers of specific diseases. This systematic review aims to collect the available evidence on the changes of SP proteome in patients exposed to OS to provide possible SP biomarkers of sperm OS. To accomplish this, the following keyterms "seminal fluid proteome", "seminal plasma proteome", "oxidative stress", and "sperm oxidative stress" were used and 137 records were found. Among these, 17 were finally included. Nine proteins involved with OS were found overexpressed in patients with OS. Twenty-three proteins were found differentially expressed in patients with clinical conditions associated with OS, such as varicocele, male accessory gland infection/inflammation, cigarette smoke, and obesity. These proteins do not seem to overlap among the clinical conditions taken into account. We speculate that specific SP proteins may mediate OS in different clinical conditions. Altogether, these results suggest that proteomics could help to better understand some of the molecular mechanisms involved in the pathogenesis of infertility. However, further studies are needed to identify potential biomarkers of male infertility with valuable clinical significance.
特发性男性不育的患病率很高，高达 75%的患者精子参数异常。因此，对其病因的研究是强制性的。氧化应激（OS）可导致 30-80%的男性不育病例。近年来，精液（SP）蛋白质组学已发展成为提供特定疾病生物标志物的有用工具。本系统评价旨在收集有关暴露于 OS 的患者 SP 蛋白质组变化的现有证据，以提供精子 OS 的可能 SP 生物标志物。为此，使用了以下关键词“精液蛋白质组”、“精液蛋白质组”、“氧化应激”和“精子氧化应激”，并找到了 137 条记录。其中，最终包括了 17 条记录。在 OS 患者中发现了 9 种与 OS 相关的蛋白质表达上调。在与 OS 相关的临床情况下，如精索静脉曲张、男性附属性腺感染/炎症、吸烟和肥胖，发现了 23 种差异表达的蛋白质。这些蛋白质似乎在考虑的临床情况下没有重叠。我们推测，特定的 SP 蛋白质可能在不同的临床情况下介导 OS。总的来说，这些结果表明蛋白质组学可能有助于更好地理解男性不育发病机制中涉及的一些分子机制。然而，需要进一步的研究来确定具有有价值临床意义的男性不育潜在生物标志物。
