Alonso R, Echeita A, Espinosa P, Usera M A
Servicio de Bacteriología, Centro Nacional de Microbiología, Madrid.
Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):579-85. doi: 10.1016/0769-2609(87)90043-3.
The notable increase in the number of Salmonella enteritidis strains seen in Spain in recent years (from 27.10% in 1980 to 66.36% in 1985) makes it necessary to find an additional epidemiological marker for this serotype. Phage typing was considered because of its discriminatory capacity toward other Salmonella serotypes. Wild and lysogenic bacteriophages were sought for a set of autochthonous bacteriophages. Our set consisted of 6 bacteriophages, 5 wild and 1 lysogenic. When tested on 1,500 selected strains, they produced 9 different phage types. The most abundant phage types were A (74.66%) and B (19.73%). The percentage of non-typable strains was low: only 1.4% from a total of 1,500 strains failed to produce lysis with our set. This reflects the high typability of the bacteriophage set proposed.
近年来在西班牙肠炎沙门氏菌菌株数量显著增加(从1980年的27.10%增至1985年的66.36%),因此有必要为该血清型寻找一个额外的流行病学标志物。鉴于噬菌体分型对其他沙门氏菌血清型的鉴别能力,故考虑采用噬菌体分型。我们寻找了一组本地噬菌体,包括野生型和溶原性噬菌体。我们的噬菌体组由6种噬菌体组成,5种野生型和1种溶原性。在对1500株选定菌株进行测试时,它们产生了9种不同的噬菌体型。最常见的噬菌体型是A(74.66%)和B(19.73%)。不可分型菌株的比例很低:在总共1500株菌株中,只有1.4%的菌株不能被我们的噬菌体组裂解。这反映了所提出的噬菌体组具有很高的分型能力。