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通过表型和基因分型方法对食源性病原体肠炎沙门氏菌爆发疫情中的菌株进行鉴别。

Differentiation of strains from a food-borne outbreak of Salmonella enterica by phenotypic and genetic typing methods.

作者信息

Gonzalez Hevia M A, Mendoza M C

机构信息

Departamento de Biología Funcional, Universidad de Oviedo, Spain.

出版信息

Eur J Epidemiol. 1995 Aug;11(4):479-82. doi: 10.1007/BF01721237.

DOI:10.1007/BF01721237
PMID:8549719
Abstract

A combination of typing methods was used to identify the strains and the infection source in a food-borne outbreak of Salmonella enterica occurring in a summer camp and affecting 25 children. All isolates tested were found to be serovar Enteritidis, with an identical biotype API 20E and ribotype. However, they differed in their plasmid profiles and/or antibiograms, and were grouped into three strains. One strain was found in human stools, another in a hen's egg, and the third in both stools and another egg pointing to large Spanish omelettes to be the contaminated food source.

摘要

采用多种分型方法对一个夏令营发生的一起沙门氏菌食源性暴发进行菌株和感染源鉴定,该暴发影响了25名儿童。所有检测的分离株均为肠炎血清型,具有相同的API 20E生物型和核糖体分型。然而,它们的质粒图谱和/或抗菌谱不同,被分为三个菌株。在人类粪便中发现了一个菌株,在一枚鸡蛋中发现了另一个菌株,在粪便和另一枚鸡蛋中均发现了第三个菌株,这表明大份西班牙煎蛋饼是受污染的食物来源。

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J Clin Microbiol. 1996 Sep;34(9):2294-6. doi: 10.1128/jcm.34.9.2294-2296.1996.
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Usefulness of ribotyping in a molecular epidemiology study of shigellosis.

本文引用的文献

1
Usefulness of molecular genetic markers in the typing of Salmonella enterica serovar Enteritidis causing a food-borne outbreak.分子遗传标记在引起食源性暴发的肠炎沙门氏菌肠炎血清型分型中的应用价值。
Int J Food Microbiol. 1994 May;22(2-3):97-103. doi: 10.1016/0168-1605(94)90134-1.
2
Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli.含有大肠杆菌rrnB核糖体RNA操纵子的重组质粒的构建与精细定位。
Plasmid. 1981 Jul;6(1):112-8. doi: 10.1016/0147-619x(81)90058-5.
3
Attempts to establish phage typing as an epidemiological marker for Salmonella enteritidis.
核糖体分型在志贺氏菌病分子流行病学研究中的应用价值
Epidemiol Infect. 1996 Apr;116(2):127-35. doi: 10.1017/s0950268800052353.
将噬菌体分型确立为肠炎沙门氏菌流行病学标志物的尝试。
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4
Possible relationship of a 36-megadalton Salmonella enteritidis plasmid to virulence in mice.一种36兆道尔顿肠炎沙门氏菌质粒与小鼠毒力之间的可能关系。
Infect Immun. 1985 Mar;47(3):831-3. doi: 10.1128/iai.47.3.831-833.1985.
5
Plasmid profiles as an epidemiological marker for Salmonella enterica serotype Enteritidis foodborne outbreaks.质粒图谱作为肠炎沙门氏菌肠炎血清型食源性暴发的流行病学标志物。
Microbiologia. 1990 Jun;6(1):45-50.
6
rRNA gene restriction patterns and plasmid analysis as a tool for typing Salmonella enteritidis.核糖体RNA基因限制性图谱和质粒分析作为肠炎沙门氏菌分型的一种工具。
Res Microbiol. 1990 Nov-Dec;141(9):1151-62. doi: 10.1016/0923-2508(90)90088-8.
7
Molecular and epidemiological study of Salmonella clinical isolates.沙门氏菌临床分离株的分子与流行病学研究
J Clin Microbiol. 1991 May;29(5):927-32. doi: 10.1128/jcm.29.5.927-932.1991.
8
Species-specific detection of Listeria monocytogenes by DNA amplification.通过DNA扩增对单核细胞增生李斯特菌进行种特异性检测。
Appl Environ Microbiol. 1991 Feb;57(2):606-9. doi: 10.1128/aem.57.2.606-609.1991.