Zhao Haidong, Wu Mingli, Liu Shirong, Tang Xiaoqin, Yi Xiaohua, Li Qi, Wang Shuhui, Sun Xiuzhu
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
College of Grassland Agriculture, Northwest A&F University, Yangling 712100, China.
Animals (Basel). 2020 Nov 22;10(11):2184. doi: 10.3390/ani10112184.
(), a highly conservative transcription factor of placental mammals, has conservative interaction of insulin-like growth factor 2 () based on the 16 bp binding sites of on the sequence. IGF2 is related to embryo growth and cell proliferation. At the same time, its functions in muscle and adipose in mammals have been widely mentioned in recent studies. To further investigate the mechanism of on , we detected the expression of and related genes in single allele knockout (-SKO) pig tissues and analyzed the transcriptome of -SKO pig liver. Through RNA-seq, we captured nine up-regulated genes and eight down-regulated genes which related to lipid metabolism. The results showed that the mRNA of had an upward trend after the partial knockout of in liver and had no significant difference in protein expression of IGF2. In summary, -SKO could affect the secretion of IGF2 in pig liver and its own lipid metabolism. Our research has provided basic information for revealing the regulatory mechanism of the interaction between ZBED6 and IGF2 in mammals.
ZBED6是胎盘哺乳动物中一种高度保守的转录因子，基于其在IGF2序列上的16个碱基对结合位点，与胰岛素样生长因子2（IGF2）存在保守的相互作用。IGF2与胚胎生长和细胞增殖有关。同时，其在哺乳动物肌肉和脂肪中的功能在最近的研究中被广泛提及。为了进一步研究ZBED6对IGF2的作用机制，我们检测了ZBED6单等位基因敲除（ZBED6-SKO）猪组织中IGF2及相关基因的表达，并分析了ZBED6-SKO猪肝的转录组。通过RNA测序，我们捕获了9个与脂质代谢相关的上调基因和8个下调基因。结果表明，肝脏中ZBED6部分敲除后，IGF2的mRNA呈上升趋势，而IGF2的蛋白表达无显著差异。综上所述，ZBED6-SKO可影响猪肝中IGF2的分泌及其自身的脂质代谢。我们的研究为揭示哺乳动物中ZBED6与IGF2相互作用的调控机制提供了基础信息。
