Sandate-Flores Luisaldo, Romero-Esquivel Eduardo, Rodríguez-Rodríguez José, Rostro-Alanis Magdalena, Melchor-Martínez Elda M, Castillo-Zacarías Carlos, Ontiveros Patricia Reyna, Celaya Marcos Fredy Morales, Chen Wei-Ning, Iqbal Hafiz M N, Parra-Saldívar Roberto
Tecnologico de Monterrey, School of Engineering and Sciences, Centro de Biotecnología FEMSA, Avenida Eugenio Garza Sada 2501, Monterrey 64849, Mexico.
Universidad Iberoamericana, Puebla, Avenida Tres Oriente, 615, 6, Centro, Puebla C.P. 72000, Mexico.
Plants (Basel). 2020 Nov 22;9(11):1623. doi: 10.3390/plants9111623.
Mexico has a great diversity of cacti, however, many of their fruits have not been studied in greater depth. Several bioactive compounds available in cacti juices extract have demonstrated nutraceutical properties. Two cactus species are interesting for their biologically active pigments, which are chico ( ()) and jiotilla ( ()). Hence, the goal of this work was to evaluate the bioactive compounds, i.e., betalains, total phenolic, vitamin C, antioxidant, and mineral content in the extract of the above-mentioned and . Then, clarified extracts were evaluated for their antioxidant activity and cytotoxicity (cancer cell lines) potentialities. Based on the obtained results, Chico fruit extract was found to be a good source of vitamin C (27.19 ± 1.95 mg L-Ascorbic acid/100 g fresh sample). Moreover, chico extract resulted in a high concentration of micronutrients, i.e., potassium (517.75 ± 16.78 mg/100 g) and zinc (2.46 ± 0.65 mg/100 g). On the other hand, Jiotilla has a high content of biologically active pigment, i.e., betaxanthins (4.17 ± 0.35 mg/g dry sample). The antioxidant activities of clarified extracts of chico and jiotilla were 80.01 ± 5.10 and 280.88 ± 7.62 mg/100 g fresh sample (DPPH method), respectively. From the cytotoxicity perspective against cancer cell lines, i.e., CaCo-2, MCF-7, HepG2, and PC-3, the clarified extracts of chico showed cytotoxicity (%cell viability) in CaCo-2 (49.7 ± 0.01%) and MCF-7 (45.56 ± 0.05%). A normal fibroblast cell line (NIH/3T3) was used, as a control, for comparison purposes. While jiotilla extract had cytotoxicity against HepG2 (47.31 ± 0.03%) and PC-3 (53.65 ± 0.04%). These results demonstrated that Chico and jiotilla are excellent resources of biologically active constituents with nutraceuticals potentialities.
墨西哥拥有种类繁多的仙人掌，然而，它们的许多果实尚未得到更深入的研究。仙人掌汁提取物中含有的几种生物活性化合物已显示出营养保健特性。有两种仙人掌因其生物活性色素而备受关注，即奇科（()）和乔蒂亚（()）。因此，本研究的目的是评估上述奇科和乔蒂亚提取物中的生物活性化合物，即甜菜色素、总酚、维生素C、抗氧化剂和矿物质含量。然后，对澄清提取物的抗氧化活性和细胞毒性（癌细胞系）潜力进行评估。根据所得结果，发现奇科果实提取物是维生素C的良好来源（27.19±1.95毫克L - 抗坏血酸/100克新鲜样品）。此外，奇科提取物含有高浓度的微量营养素，即钾（517.75±16.78毫克/100克）和锌（2.46±0.65毫克/100克）。另一方面，乔蒂亚含有高含量的生物活性色素，即甜菜黄素（4.17±0.35毫克/克干样品）。奇科和乔蒂亚澄清提取物的抗氧化活性分别为80.01±5.10和280.88±7.62毫克/100克新鲜样品（DPPH法）。从对癌细胞系（即CaCo - 2、MCF - 7、HepG2和PC - 3）的细胞毒性角度来看，奇科的澄清提取物在CaCo - 2（49.7±0.01%）和MCF - 7（45.56±0.05%）中显示出细胞毒性（细胞活力百分比）。作为对照，使用正常成纤维细胞系（NIH/3T3）进行比较。而乔蒂亚提取物对HepG2（47.31±0.03%）和PC - 3（53.65±0.04%）具有细胞毒性。这些结果表明，奇科和乔蒂亚是具有营养保健潜力的生物活性成分的优质来源。
